Salivary antioxidants status following progressive aerobic exercise: What are the differences between waterpipe smokers and non-smokers?

Hamid Arazi, Behzad Taati, Forough Rafati Sajedi, Katsuhiko Suzuki

Research output: Contribution to journalArticle

Abstract

Waterpipe tobacco (WPT) smoking is a public health problem with similar or even stronger effects than cigarette smoking. Although it appears to be associated with extensive oxidative stress, there is a limited number of studies on the oxidative effects of WPT smoking in stressful conditions. We, therefore, compared the responses of salivary flow rate (SFR), uric acid (UA) concentration, and peroxidase (POX) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) activities between WPT smokers and non-smokers following a bout of exhaustive aerobic exercise (AE). Twenty-three sedentary young women (age: 22.95 ± 2.83 years) participated in this study, including 11 smokers (7.00 ± 1.41 uses/week) and 12 non-smokers. All participants were required to perform the Bruce protocol treadmill test at an initial gradient of 10% at 1.7 mph, with increases of these parameters every 3 min until exhaustion. Unstimulated saliva samples were collected before, immediately after, and 1 hour after AE. WPT smokers showed lower SFR compared with non-smokers at all time points (p < 0.05). In comparison to WPT smokers, a larger increase in POX activity (approximately 23% vs 14%; p = 0.009) and a smaller decline in DPPH activity (approximately −8% vs −15%; p = 0.004) were found in non-smokers compared with WPT smokers. While these changes were slowly compensated within 1 hour after exhaustion, the activity of both markers was different from the pre-exercise values (p < 0.001). There was also a trend for UA concentration in non-smokers to be higher during the recovery period, with no significant difference between the groups (p > 0.05). It seems that WPT smoking is associated with negative effects on important human antioxidants and a diminished antioxidative response following acute exercise.

Original languageEnglish
Article number418
JournalAntioxidants
Volume8
Issue number10
DOIs
Publication statusPublished - 2019 Oct

Fingerprint

Tobacco
Antioxidants
Smoking
Exercise
Flow rate
Exercise equipment
Oxidative stress
Public health
Medical problems
Uric Acid
Hydrates
Tobacco Products
Peroxidase
Exercise Test
Saliva
Oxidative Stress
Public Health

Keywords

  • DPPH
  • Free radicals
  • Hookah
  • Peroxidase
  • Saliva
  • Uric acid

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Salivary antioxidants status following progressive aerobic exercise : What are the differences between waterpipe smokers and non-smokers? / Arazi, Hamid; Taati, Behzad; Sajedi, Forough Rafati; Suzuki, Katsuhiko.

In: Antioxidants, Vol. 8, No. 10, 418, 10.2019.

Research output: Contribution to journalArticle

@article{bb26f386f0ae4e82b09b430f71304d03,
title = "Salivary antioxidants status following progressive aerobic exercise: What are the differences between waterpipe smokers and non-smokers?",
abstract = "Waterpipe tobacco (WPT) smoking is a public health problem with similar or even stronger effects than cigarette smoking. Although it appears to be associated with extensive oxidative stress, there is a limited number of studies on the oxidative effects of WPT smoking in stressful conditions. We, therefore, compared the responses of salivary flow rate (SFR), uric acid (UA) concentration, and peroxidase (POX) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) activities between WPT smokers and non-smokers following a bout of exhaustive aerobic exercise (AE). Twenty-three sedentary young women (age: 22.95 ± 2.83 years) participated in this study, including 11 smokers (7.00 ± 1.41 uses/week) and 12 non-smokers. All participants were required to perform the Bruce protocol treadmill test at an initial gradient of 10{\%} at 1.7 mph, with increases of these parameters every 3 min until exhaustion. Unstimulated saliva samples were collected before, immediately after, and 1 hour after AE. WPT smokers showed lower SFR compared with non-smokers at all time points (p < 0.05). In comparison to WPT smokers, a larger increase in POX activity (approximately 23{\%} vs 14{\%}; p = 0.009) and a smaller decline in DPPH activity (approximately −8{\%} vs −15{\%}; p = 0.004) were found in non-smokers compared with WPT smokers. While these changes were slowly compensated within 1 hour after exhaustion, the activity of both markers was different from the pre-exercise values (p < 0.001). There was also a trend for UA concentration in non-smokers to be higher during the recovery period, with no significant difference between the groups (p > 0.05). It seems that WPT smoking is associated with negative effects on important human antioxidants and a diminished antioxidative response following acute exercise.",
keywords = "DPPH, Free radicals, Hookah, Peroxidase, Saliva, Uric acid",
author = "Hamid Arazi and Behzad Taati and Sajedi, {Forough Rafati} and Katsuhiko Suzuki",
year = "2019",
month = "10",
doi = "10.3390/antiox8100418",
language = "English",
volume = "8",
journal = "Antioxidants",
issn = "2076-3921",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "10",

}

TY - JOUR

T1 - Salivary antioxidants status following progressive aerobic exercise

T2 - What are the differences between waterpipe smokers and non-smokers?

AU - Arazi, Hamid

AU - Taati, Behzad

AU - Sajedi, Forough Rafati

AU - Suzuki, Katsuhiko

PY - 2019/10

Y1 - 2019/10

N2 - Waterpipe tobacco (WPT) smoking is a public health problem with similar or even stronger effects than cigarette smoking. Although it appears to be associated with extensive oxidative stress, there is a limited number of studies on the oxidative effects of WPT smoking in stressful conditions. We, therefore, compared the responses of salivary flow rate (SFR), uric acid (UA) concentration, and peroxidase (POX) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) activities between WPT smokers and non-smokers following a bout of exhaustive aerobic exercise (AE). Twenty-three sedentary young women (age: 22.95 ± 2.83 years) participated in this study, including 11 smokers (7.00 ± 1.41 uses/week) and 12 non-smokers. All participants were required to perform the Bruce protocol treadmill test at an initial gradient of 10% at 1.7 mph, with increases of these parameters every 3 min until exhaustion. Unstimulated saliva samples were collected before, immediately after, and 1 hour after AE. WPT smokers showed lower SFR compared with non-smokers at all time points (p < 0.05). In comparison to WPT smokers, a larger increase in POX activity (approximately 23% vs 14%; p = 0.009) and a smaller decline in DPPH activity (approximately −8% vs −15%; p = 0.004) were found in non-smokers compared with WPT smokers. While these changes were slowly compensated within 1 hour after exhaustion, the activity of both markers was different from the pre-exercise values (p < 0.001). There was also a trend for UA concentration in non-smokers to be higher during the recovery period, with no significant difference between the groups (p > 0.05). It seems that WPT smoking is associated with negative effects on important human antioxidants and a diminished antioxidative response following acute exercise.

AB - Waterpipe tobacco (WPT) smoking is a public health problem with similar or even stronger effects than cigarette smoking. Although it appears to be associated with extensive oxidative stress, there is a limited number of studies on the oxidative effects of WPT smoking in stressful conditions. We, therefore, compared the responses of salivary flow rate (SFR), uric acid (UA) concentration, and peroxidase (POX) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) activities between WPT smokers and non-smokers following a bout of exhaustive aerobic exercise (AE). Twenty-three sedentary young women (age: 22.95 ± 2.83 years) participated in this study, including 11 smokers (7.00 ± 1.41 uses/week) and 12 non-smokers. All participants were required to perform the Bruce protocol treadmill test at an initial gradient of 10% at 1.7 mph, with increases of these parameters every 3 min until exhaustion. Unstimulated saliva samples were collected before, immediately after, and 1 hour after AE. WPT smokers showed lower SFR compared with non-smokers at all time points (p < 0.05). In comparison to WPT smokers, a larger increase in POX activity (approximately 23% vs 14%; p = 0.009) and a smaller decline in DPPH activity (approximately −8% vs −15%; p = 0.004) were found in non-smokers compared with WPT smokers. While these changes were slowly compensated within 1 hour after exhaustion, the activity of both markers was different from the pre-exercise values (p < 0.001). There was also a trend for UA concentration in non-smokers to be higher during the recovery period, with no significant difference between the groups (p > 0.05). It seems that WPT smoking is associated with negative effects on important human antioxidants and a diminished antioxidative response following acute exercise.

KW - DPPH

KW - Free radicals

KW - Hookah

KW - Peroxidase

KW - Saliva

KW - Uric acid

UR - http://www.scopus.com/inward/record.url?scp=85073412404&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073412404&partnerID=8YFLogxK

U2 - 10.3390/antiox8100418

DO - 10.3390/antiox8100418

M3 - Article

AN - SCOPUS:85073412404

VL - 8

JO - Antioxidants

JF - Antioxidants

SN - 2076-3921

IS - 10

M1 - 418

ER -