Sarcomere length-dependent Ca 2+ activation in skinned rabbit psoas muscle fibers: Coordinated regulation of thin filament cooperative activation and passive force

Norio Fukuda, Takahiro Inoue, Mitsunori Yamane, Takako Terui, Fuyu Kobirumaki, Iwao Ohtsuki, Shin'ichi Ishiwata, Satoshi Kurihara

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    In skeletal muscle, active force production varies as a function of sarcomere length (SL). It has been considered that this SL dependence results simply from a change in the overlap length between the thick and thin filaments. The purpose of this study was to provide a systematic understanding of the SL-dependent increase in Ca 2+ sensitivity in skeletal muscle, by investigating how thin filament "on-off" switching and passive force are involved in the regulation. Rabbit psoas muscles were skinned, and active force measurements were taken at various Ca 2+ concentrations with single fibers, in the short (2.0 and 2.4 μm) and long (2.4 and 2.8 μm) SL ranges. Despite the same magnitude of SL elongation, the SLdependent increase in Ca 2+ sensitivity was more pronounced in the long SL range. MgADP (3 mM) increased the rate of rise of active force and attenuated SL-dependent Ca 2+ activation in both SL ranges. Conversely, inorganic phosphate (Pi, 20 mM) decreased the rate of rise of active force and enhanced SL-dependent Ca 2+ activation in both SL ranges. Our analyses revealed that, in the absence and presence of MgADP or Pi, the magnitude of SL-dependent Ca 2+ activation was (1) inversely correlated with the rate of rise of active force, and (2) in proportion to passive force. These findings suggest that the SL dependence of active force in skeletal muscle is regulated via thin filament "on-off" switching and titin (connectin)-based interfilament lattice spacing modulation in a coordinated fashion, in addition to the regulation via the filament overlap.

    Original languageEnglish
    Pages (from-to)515-523
    Number of pages9
    JournalJournal of Physiological Sciences
    Volume61
    Issue number6
    DOIs
    Publication statusPublished - 2011 Nov

    Fingerprint

    Psoas Muscles
    Sarcomeres
    Rabbits
    Connectin
    Skeletal Muscle
    Adenosine Diphosphate

    Keywords

    • Ca
    • Muscle mechanics
    • Sensitivity
    • Titin
    • Troponin

    ASJC Scopus subject areas

    • Physiology

    Cite this

    Sarcomere length-dependent Ca 2+ activation in skinned rabbit psoas muscle fibers : Coordinated regulation of thin filament cooperative activation and passive force. / Fukuda, Norio; Inoue, Takahiro; Yamane, Mitsunori; Terui, Takako; Kobirumaki, Fuyu; Ohtsuki, Iwao; Ishiwata, Shin'ichi; Kurihara, Satoshi.

    In: Journal of Physiological Sciences, Vol. 61, No. 6, 11.2011, p. 515-523.

    Research output: Contribution to journalArticle

    Fukuda, Norio ; Inoue, Takahiro ; Yamane, Mitsunori ; Terui, Takako ; Kobirumaki, Fuyu ; Ohtsuki, Iwao ; Ishiwata, Shin'ichi ; Kurihara, Satoshi. / Sarcomere length-dependent Ca 2+ activation in skinned rabbit psoas muscle fibers : Coordinated regulation of thin filament cooperative activation and passive force. In: Journal of Physiological Sciences. 2011 ; Vol. 61, No. 6. pp. 515-523.
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