SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates

Shin ya Okamoto, Masamitsu Sato, Takashi Toda, Masayuki; Yamamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The SCF (Skp1-Cul1-F-box) complex contributes to a variety of cellular events including meiotic cell cycle control, but its function during meiosis is not understood well. Here we describe a novel function of SCF/Skp1 in meiotic recombination and subsequent chromosome segregation. The skp1 temperature-sensitive mutant exhibited abnormal distribution of spindle microtubules in meiosis II, which turned out to originate from abnormal bending of the spindle in meiosis I. Bent spindles were reported in mitosis of this mutant, but it remained unknown how SCF could affect spindle morphology. We found that the meiotic bent spindle in skp1 cells was due to a hypertension generated by chromosome entanglement. The spindle bending was suppressed by inhibiting double strand break (DSB) formation, indicating that the entanglement was generated by the meiotic recombination machinery. Consistently, Rhp51/Rad51-Rad22/Rad52 foci persisted until meiosis I in skp1 cells, proving accumulation of recombination intermediates. Intriguingly bent spindles were also observed in the mutant of Fbh1, an F-box protein containing the DNA helicase domain, which is involved in meiotic recombination. Genetic evidence suggested its cooperation with SCF/Skp1. Thus, SCF/Skp1 together with Fbh1 is likely to function in the resolution of meiotic recombination intermediates, thereby ensuring proper chromosome segregation.

Original languageEnglish
Article numbere30622
JournalPLoS One
Volume7
Issue number1
DOIs
Publication statusPublished - 2012 Jan 23
Externally publishedYes

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Chromosome Segregation
chromosome segregation
Chromosomes
meiosis
Genetic Recombination
Meiosis
F-Box Proteins
mutants
DNA Helicases
DNA helicases
F-box proteins
Machinery
Cells
Spindle Apparatus
mitosis
hypertension
microtubules
cell cycle
Cell Cycle Checkpoints
Mitosis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates. / Okamoto, Shin ya; Sato, Masamitsu; Toda, Takashi; Yamamoto, Masayuki;.

In: PLoS One, Vol. 7, No. 1, e30622, 23.01.2012.

Research output: Contribution to journalArticle

Okamoto, Shin ya ; Sato, Masamitsu ; Toda, Takashi ; Yamamoto, Masayuki;. / SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates. In: PLoS One. 2012 ; Vol. 7, No. 1.
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