Screening and characterization of a novel reversible 4-hydroxyisophthalic acid decarboxylase from Cystobasidium slooffiae HTK3

Riku Aono, Tomoya Yoshihara, Hotaka Nishida, Kuniki Kino

Research output: Contribution to journalArticlepeer-review

Abstract

Owing to carboxylation activity, reversible decarboxylases can use CO2 as a C1-building block to produce useful carboxylic acids. Although many reversible decarboxylases can synthesize aromatic monocarboxylic acids, only a few reversible decarboxylases have been reported to date that catalyze the synthesis of aromatic dicarboxylic acids. In the present study, a reversible 4-hydroxyisophthalic acid decarboxylase was identified in Cystobasidium slooffiae HTK3. Furthermore, recombinant 4-hydroxyisophthalic acid decarboxylase was prepared, characterized, and used for 4-hydroxyisophthalic acid production from 4-hydroxybenzoic acid.

Original languageEnglish
Pages (from-to)1658-1664
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume85
Issue number7
DOIs
Publication statusPublished - 2021 Jul 1

Keywords

  • aromatic dicarboxylic acid
  • microbial screening
  • reversible decarboxylase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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