Screening of mutants using chlorophyll fluorescence

Takako Ogawa, Kintake Sonoike*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Chlorophyll fluorescence has been widely used for the estimation of photosynthesis or its regulatory mechanisms. Chlorophyll fluorescence measurements are the methods with non-destructive nature and do not require contact between plant materials and fluorometers. Furthermore, the measuring process is very rapid. These characteristics of chlorophyll fluorescence measurements make them a suitable tool to screen mutants of photosynthesis-related genes. Furthermore, it has been shown that genes with a wide range of functions can be also analyzed by chlorophyll fluorescence through metabolic interactions. In this short review, we would like to first introduce the basic principle of the chlorophyll fluorescence measurements, and then explore the advantages and limitation of various screening methods. The emphasis is on the possibility of chlorophyll fluorescence measurements to screen mutants defective in metabolisms other than photosynthesis.

Original languageEnglish
Pages (from-to)653-664
Number of pages12
JournalJournal of Plant Research
Volume134
Issue number4
DOIs
Publication statusPublished - 2021 Jul

Keywords

  • Chlorophyll fluorescence
  • Imaging
  • Kautsky transient
  • Metabolic interaction
  • OJIP analysis
  • Screening

ASJC Scopus subject areas

  • Plant Science

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