Screening of soil bacteria for production of biocleaner

Haruko Takeyama, Masumi Wada, Tadashi Matsunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Soil bacteria were studied for the production of biodegradable cleaning agents. Among 86 bacterial strains resistant to liquid paraffin, 58 showed hemolytic activity. These strains were cultured, and the supernatant of culture broths was evaluated for cleaning activity against a dirty porcelain tile. Potent activity was exhibited in 18 strains. The lowest value of surface tension was obtained from Bacillus sp. NKB03 suggesting the presence of a biosurfactant. Aeromonas sp. NKB26c and Bacillus cereus NKB46b exhibited enzymatic cleaning activity. A cleaning efficiency of 82% was achieved when using a mixture of supernatants from culture broths of Bacillus sp. NKB03 and Aeromonas sp. NKB26c in synthetic minimal media. The cleaning efficiency using this mixture was higher than that of sodium dodecyl sulfate. These results suggest that a mixture of supernatants from culture broths of Bacillus sp. NKB03 and Aeromonas sp. NKB26c has potential for commercial use as a biocleaner.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume98-100
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Aeromonas
Bacillus
Cleaning
Bacteria
Screening
Soil
Soils
Bacilli
Mineral Oil
Dental Porcelain
Surface Tension
Bacillus cereus
Sodium Dodecyl Sulfate
Porcelain
Sodium dodecyl sulfate
Tile
Paraffins
Surface tension
Liquids

Keywords

  • Biocleaner
  • Biosurfactant
  • Hemolytic activity
  • NIHImage analysis
  • Soil bacteria

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Screening of soil bacteria for production of biocleaner. / Takeyama, Haruko; Wada, Masumi; Matsunaga, Tadashi.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 98-100, 2002, p. 319-326.

Research output: Contribution to journalArticle

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