Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish

Hitoshi Ohmori, Masayuki Raitoh, Mikiya Harano, Toshiyuki Oda, Masaki Hikida, Ryosuke Sugihara, Kazunaga Yazawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Culture supernatants of enteric bacteria isolated from the gastrointestinal tracts of two marine fishes were screened for the production of immunosuppressive substances. At least 50% of the 60 bacterial isolates tested were found to produce immunosuppressants that significantly inhibited the induction of cytotoxic T cells (CTL) in vitro. The strains that secreted the highest suppressive activity was identified as Aeromonas caviae. The immunosuppressive substance purified from the A. caviae culture supernatant was a polysaccharide with a molecular weight of about 70 kDa that contained mannose : glucose (4:1). This polysaccharide did not show nonspecific cytotoxicity, and was found to inhibit the proliferation and differentiation of CD8-positive CTL.

Original languageEnglish
Pages (from-to)117-119
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume85
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

T-cells
Aeromonas caviae
Enterobacteriaceae
Immunosuppressive Agents
Polysaccharides
Fish
Bacteria
Fishes
Cytotoxicity
T-Lymphocytes
Glucose
Molecular weight
Mannose
Gastrointestinal Tract
Molecular Weight

Keywords

  • Cytotoxic T cells
  • Fish enteric bacteria
  • Immune suppression
  • Polysaccharides

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish. / Ohmori, Hitoshi; Raitoh, Masayuki; Harano, Mikiya; Oda, Toshiyuki; Hikida, Masaki; Sugihara, Ryosuke; Yazawa, Kazunaga.

In: Journal of Fermentation and Bioengineering, Vol. 85, No. 1, 1998, p. 117-119.

Research output: Contribution to journalArticle

Ohmori, Hitoshi ; Raitoh, Masayuki ; Harano, Mikiya ; Oda, Toshiyuki ; Hikida, Masaki ; Sugihara, Ryosuke ; Yazawa, Kazunaga. / Search for immunosuppressive substances produced in the culture of enteric bacteria isolated from marine fish. In: Journal of Fermentation and Bioengineering. 1998 ; Vol. 85, No. 1. pp. 117-119.
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AU - Hikida, Masaki

AU - Sugihara, Ryosuke

AU - Yazawa, Kazunaga

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