Abstract
Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
Original language | English |
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Pages (from-to) | 1847-1852 |
Number of pages | 6 |
Journal | Biotechnology Letters |
Volume | 33 |
Issue number | 9 |
DOIs | |
Publication status | Published - 2011 Sep |
Externally published | Yes |
Keywords
- Bacillus subtilis
- Human interferon-β
- Propeptide
- Protein secretion
- PrsA
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology