Abstract
Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
| Original language | English |
|---|---|
| Pages (from-to) | 1847-1852 |
| Number of pages | 6 |
| Journal | Biotechnology Letters |
| Volume | 33 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2011 Sep 1 |
| Externally published | Yes |
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Keywords
- Bacillus subtilis
- Human interferon-β
- Propeptide
- Protein secretion
- PrsA
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Secretion of biologically-active human interferon-β by Bacillus subtilis. / Kakeshita, Hiroshi; Kageyama, Yasushi; Endo, Keiji; Tohata, Masatoshi; Ara, Katsutoshi; Ozaki, Katsuya; Nakamura, Kouji.
In: Biotechnology Letters, Vol. 33, No. 9, 01.09.2011, p. 1847-1852.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Secretion of biologically-active human interferon-β by Bacillus subtilis
AU - Kakeshita, Hiroshi
AU - Kageyama, Yasushi
AU - Endo, Keiji
AU - Tohata, Masatoshi
AU - Ara, Katsutoshi
AU - Ozaki, Katsuya
AU - Nakamura, Kouji
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
AB - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.
KW - Bacillus subtilis
KW - Human interferon-β
KW - Propeptide
KW - Protein secretion
KW - PrsA
UR - http://www.scopus.com/inward/record.url?scp=80051475096&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80051475096&partnerID=8YFLogxK
U2 - 10.1007/s10529-011-0636-2
DO - 10.1007/s10529-011-0636-2
M3 - Article
C2 - 21544609
AN - SCOPUS:80051475096
VL - 33
SP - 1847
EP - 1852
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 9
ER -