Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

Original languageEnglish
Pages (from-to)1847-1852
Number of pages6
JournalBiotechnology Letters
Volume33
Issue number9
DOIs
Publication statusPublished - 2011 Sep 1
Externally publishedYes

Keywords

  • Bacillus subtilis
  • Human interferon-β
  • Propeptide
  • Protein secretion
  • PrsA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • Cite this

    Kakeshita, H., Kageyama, Y., Endo, K., Tohata, M., Ara, K., Ozaki, K., & Nakamura, K. (2011). Secretion of biologically-active human interferon-β by Bacillus subtilis. Biotechnology Letters, 33(9), 1847-1852. https://doi.org/10.1007/s10529-011-0636-2