Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita; Yasushi Kageyama; Keiji Endo; Masatoshi Tohata; Katsutoshi Ara; Katsuya Ozaki; Kouji Nakamura

doi:10.1007/s10529-011-0636-2

Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita^*, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

Original language	English
Pages (from-to)	1847-1852
Number of pages	6
Journal	Biotechnology Letters
Volume	33
Issue number	9
DOIs	https://doi.org/10.1007/s10529-011-0636-2
Publication status	Published - 2011 Sep
Externally published	Yes

Keywords

Bacillus subtilis
Human interferon-β
Propeptide
Protein secretion
PrsA

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-011-0636-2

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title = "Secretion of biologically-active human interferon-β by Bacillus subtilis",

abstract = "Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.",

keywords = "Bacillus subtilis, Human interferon-β, Propeptide, Protein secretion, PrsA",

author = "Hiroshi Kakeshita and Yasushi Kageyama and Keiji Endo and Masatoshi Tohata and Katsutoshi Ara and Katsuya Ozaki and Kouji Nakamura",

note = "Funding Information: Acknowledgments This work is the subproject, {\textquoteleft}Development of a Technology for Creation of a Host Cell{\textquoteright} included within the industrial technology project, {\textquoteleft}Development of a Generic Technology for Production Process Starting Productive Function{\textquoteright} of the Ministry of Economy, Trade and Industry, entrusted by the New Energy and Industrial Technology Development Organization (NEDO), Japan. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2011",

month = sep,

doi = "10.1007/s10529-011-0636-2",

language = "English",

volume = "33",

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AU - Kakeshita, Hiroshi

AU - Kageyama, Yasushi

AU - Endo, Keiji

AU - Tohata, Masatoshi

AU - Ara, Katsutoshi

AU - Ozaki, Katsuya

AU - Nakamura, Kouji

N1 - Funding Information: Acknowledgments This work is the subproject, ‘Development of a Technology for Creation of a Host Cell’ included within the industrial technology project, ‘Development of a Generic Technology for Production Process Starting Productive Function’ of the Ministry of Economy, Trade and Industry, entrusted by the New Energy and Industrial Technology Development Organization (NEDO), Japan. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2011/9

Y1 - 2011/9

N2 - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

AB - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

KW - Bacillus subtilis

KW - Human interferon-β

KW - Propeptide

KW - Protein secretion

KW - PrsA

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JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 9

ER -

Secretion of biologically-active human interferon-β by Bacillus subtilis

Abstract

Keywords

ASJC Scopus subject areas

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