Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

Original languageEnglish
Pages (from-to)1847-1852
Number of pages6
JournalBiotechnology Letters
Volume33
Issue number9
DOIs
Publication statusPublished - 2011 Sep 1
Externally publishedYes

Fingerprint

Interferons
Bacilli
Bacillus subtilis
Human Activities
Proteins

Keywords

  • Bacillus subtilis
  • Human interferon-β
  • Propeptide
  • Protein secretion
  • PrsA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Kakeshita, H., Kageyama, Y., Endo, K., Tohata, M., Ara, K., Ozaki, K., & Nakamura, K. (2011). Secretion of biologically-active human interferon-β by Bacillus subtilis. Biotechnology Letters, 33(9), 1847-1852. https://doi.org/10.1007/s10529-011-0636-2

Secretion of biologically-active human interferon-β by Bacillus subtilis. / Kakeshita, Hiroshi; Kageyama, Yasushi; Endo, Keiji; Tohata, Masatoshi; Ara, Katsutoshi; Ozaki, Katsuya; Nakamura, Kouji.

In: Biotechnology Letters, Vol. 33, No. 9, 01.09.2011, p. 1847-1852.

Research output: Contribution to journalArticle

Kakeshita, H, Kageyama, Y, Endo, K, Tohata, M, Ara, K, Ozaki, K & Nakamura, K 2011, 'Secretion of biologically-active human interferon-β by Bacillus subtilis', Biotechnology Letters, vol. 33, no. 9, pp. 1847-1852. https://doi.org/10.1007/s10529-011-0636-2
Kakeshita, Hiroshi ; Kageyama, Yasushi ; Endo, Keiji ; Tohata, Masatoshi ; Ara, Katsutoshi ; Ozaki, Katsuya ; Nakamura, Kouji. / Secretion of biologically-active human interferon-β by Bacillus subtilis. In: Biotechnology Letters. 2011 ; Vol. 33, No. 9. pp. 1847-1852.
@article{fa57de1684964421b2a9a5a6e25182c4,
title = "Secretion of biologically-active human interferon-β by Bacillus subtilis",
abstract = "Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.",
keywords = "Bacillus subtilis, Human interferon-β, Propeptide, Protein secretion, PrsA",
author = "Hiroshi Kakeshita and Yasushi Kageyama and Keiji Endo and Masatoshi Tohata and Katsutoshi Ara and Katsuya Ozaki and Kouji Nakamura",
year = "2011",
month = "9",
day = "1",
doi = "10.1007/s10529-011-0636-2",
language = "English",
volume = "33",
pages = "1847--1852",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "9",

}

TY - JOUR

T1 - Secretion of biologically-active human interferon-β by Bacillus subtilis

AU - Kakeshita, Hiroshi

AU - Kageyama, Yasushi

AU - Endo, Keiji

AU - Tohata, Masatoshi

AU - Ara, Katsutoshi

AU - Ozaki, Katsuya

AU - Nakamura, Kouji

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

AB - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

KW - Bacillus subtilis

KW - Human interferon-β

KW - Propeptide

KW - Protein secretion

KW - PrsA

UR - http://www.scopus.com/inward/record.url?scp=80051475096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051475096&partnerID=8YFLogxK

U2 - 10.1007/s10529-011-0636-2

DO - 10.1007/s10529-011-0636-2

M3 - Article

C2 - 21544609

AN - SCOPUS:80051475096

VL - 33

SP - 1847

EP - 1852

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 9

ER -