Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita; Yasushi Kageyama; Keiji Endo; Masatoshi Tohata; Katsutoshi Ara; Katsuya Ozaki; Kouji Nakamura

doi:10.1007/s10529-011-0636-2

Secretion of biologically-active human interferon-β by Bacillus subtilis

Hiroshi Kakeshita, Yasushi Kageyama, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Kouji Nakamura

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

Original language	English
Pages (from-to)	1847-1852
Number of pages	6
Journal	Biotechnology Letters
Volume	33
Issue number	9
DOIs	https://doi.org/10.1007/s10529-011-0636-2
Publication status	Published - 2011 Sep 1
Externally published	Yes

Keywords

Bacillus subtilis
Human interferon-β
Propeptide
Protein secretion
PrsA

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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abstract = "Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.",

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T1 - Secretion of biologically-active human interferon-β by Bacillus subtilis

AU - Kakeshita, Hiroshi

AU - Kageyama, Yasushi

AU - Endo, Keiji

AU - Tohata, Masatoshi

AU - Ara, Katsutoshi

AU - Ozaki, Katsuya

AU - Nakamura, Kouji

PY - 2011/9/1

Y1 - 2011/9/1

N2 - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

AB - Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.

KW - Bacillus subtilis

KW - Human interferon-β

KW - Propeptide

KW - Protein secretion

KW - PrsA

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