Selection of mutants of Aspergillus niger showing enhanced productivity of citric acid from starch in shaking culture

Sugima Rugsaseel, Kotaro Kirimura, Shoji Usami

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Mutants with enhanced citric acid production from soluble starch were induced from Aspergillus niger WU-2223L. After UV-irradiation of a conidial suspension of strain WU-2223L, mutants were selected on modified starch-methyl red agar plates on the basis of higher amylolytic activity and acid productivity. The 8 mutants selected showed enhanced citric acid production from soluble starch in shaking culture. Among them, a representative mutant strain, 2M-43, produced 48.0gg/l of citric acid from 120 g/l of soluble starch in 9 d of cultivation in shaking culture, whereas strain WU-2223L produced 35.1 g/l. Glucoamylase activities in the culture filtrates of strains 2M-43 and WU-2223L reached maximum levels of 3.62 U/ml and 2.11 U/ml, respectively, both at 3 d of cultivation, and thereafter decreased.

    Original languageEnglish
    Pages (from-to)226-228
    Number of pages3
    JournalJournal of Fermentation and Bioengineering
    Volume75
    Issue number3
    DOIs
    Publication statusPublished - 1993

    Fingerprint

    Aspergillus niger
    Aspergillus
    Citric acid
    Starch
    Citric Acid
    Productivity
    Glucan 1,4-alpha-Glucosidase
    Agar
    Suspensions
    Irradiation
    Acids

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Selection of mutants of Aspergillus niger showing enhanced productivity of citric acid from starch in shaking culture. / Rugsaseel, Sugima; Kirimura, Kotaro; Usami, Shoji.

    In: Journal of Fermentation and Bioengineering, Vol. 75, No. 3, 1993, p. 226-228.

    Research output: Contribution to journalArticle

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