Selective ablation of an astroglial subset by toxic gene expression driven by tenascin promoter

Hiroko Ikeshima-Kataoka, Shigeki Yuasa

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4 Citations (Scopus)

Abstract

Objective: In vitro culture, one of the astroglia-derived extracellular matrix proteins, tenascin, expressed highly in fibrous astrocytes, whereas it expressed only low levels in protoplasmic astrocytes. We devised a method of selectively altering the population of astroglial subsets in primary culture of astrocytes derived from embryonic mouse brains using toxic gene expression driven by the tenascin promoter. Methods: We have identified that the segment of 512-bases of 5′-flanking plus 243-bases leader sequences of the mouse tenascin gene contains maximum promoter activity in primary culture of astrocytes by deletion analysis of 5′-upstream region. This promoter element was used to specifically express the herpes simplex virus thymidine kinase (HSV-TK) gene in tenascin-positive astrocytes. Results: This strategy allowed us to selectively decrease tenascin-positive astrocytes at the optimal concentration of ganciclovir, which is cytotoxic in HSV-TK-expressing cells. Discussion: This approach should be useful for examining the role of the tenascin-negative astroglial subset in the development and regeneration of the central nervous system.

Original languageEnglish
Pages (from-to)701-709
Number of pages9
JournalNeurological Research
Volume30
Issue number7
DOIs
Publication statusPublished - 2008 Sep
Externally publishedYes

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Keywords

  • Astroglia
  • Ganciclovir
  • Herpes simplex virus thymidine kinase
  • Promoter
  • Tenascin

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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