Selective and continuous degradation of carbazole contained in petroleum oil by resting cells of Sphingomonas sp. CDH-7

Kotaro Kirimura, Hiroyuki Nakagawa, Kenji Tsuji, Kazuya Matsuda, Ryuichiro Kurane, Shoji Usami

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    Microbial degradation of carbazole (CA), a model of hard-removal heterocyclic nitrogen compounds contained in petroleum oil, was examined using Sphingomonas sp. CDH-7 isolated from a soil sample by screening for CA-as-similating microorganisms. CDH-7 used CA as a sole source of carbon and nitrogen, and metabolized CA to ammonia via anthranilic acid as an intermediate product. When CDH-7 was cultivated in the medium containing CA at the concentration of 500 mg/1 (2.99 mM), CA was completely degraded within 50 h. By the reaction with the resting cells of CDH-7, 500 mg/1 of CA was completely degraded within 4 h, with 1.64 mM of ammonia accumulated in the reaction mixture. When CA was added at the concentration of 100 mg/1 (0.599 mM) periodically to the reaction mixture ten times, 925 mg/1 (5.54 mM) of CA was degraded within 48 h by the resting cells, and 4.50 mM of ammonia was accumulated in the reaction mixture with a 75.1% molar conversion yield based on total CA added. The resting cells could almost completely degrade CA in a two-liquid-phase system which consists of water and organic solvent, even in the presence of 20% (v/v) isooctane, n-hexane, cyciohexane, and kerosene as a model petroleum oil. In the presence of an organic solvent system such as 20% (v/v)p-xylene, toluene, and heptanol, however, CA degradation yields decreased.

    Original languageEnglish
    Pages (from-to)1563-1568
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume63
    Issue number9
    Publication statusPublished - 1999 Sep

    Fingerprint

    carbazoles
    Sphingomonas
    Petroleum
    petroleum
    Ammonia
    Oils
    Crude oil
    Degradation
    oils
    Organic solvents
    degradation
    Nitrogen compounds
    Kerosene
    cells
    Xylene
    Hexane
    Microorganisms
    Toluene
    Screening
    Nitrogen

    Keywords

    • Biodegradation
    • Carbazole
    • Heterocyclic nitrogen compound
    • Resting cell reaction
    • Sphingomonas sp.

    ASJC Scopus subject areas

    • Food Science
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology
    • Chemistry (miscellaneous)
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Selective and continuous degradation of carbazole contained in petroleum oil by resting cells of Sphingomonas sp. CDH-7. / Kirimura, Kotaro; Nakagawa, Hiroyuki; Tsuji, Kenji; Matsuda, Kazuya; Kurane, Ryuichiro; Usami, Shoji.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 9, 09.1999, p. 1563-1568.

    Research output: Contribution to journalArticle

    Kirimura, Kotaro ; Nakagawa, Hiroyuki ; Tsuji, Kenji ; Matsuda, Kazuya ; Kurane, Ryuichiro ; Usami, Shoji. / Selective and continuous degradation of carbazole contained in petroleum oil by resting cells of Sphingomonas sp. CDH-7. In: Bioscience, Biotechnology and Biochemistry. 1999 ; Vol. 63, No. 9. pp. 1563-1568.
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