Selective detection of Escherichia coli by imaging of the light intensity transmitted through an optical disk

Hideyuki Shiramizu, Chiaki Kuroda, Yoshimichi Ohki, Takayuki Shima, Xiaomin Wang, Makoto Fujimaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We have developed an optical disk system for imaging transmitted light from Escherichia coli dispersed on an optical disk. When E. coli was stained using Bismarck brown, the transmittance was found to decrease in images obtained at λ = 405 nm. The results indicate that transmittance imaging is suitable for finding the difference in light intensity between stained and unstained E. coli, whereas the reflectance images were scarcely changed by staining. Therefore, E. coli can be selectively discriminated from abiotic contaminants using transmittance imaging.

Original languageEnglish
Article number037001
JournalApplied Physics Express
Volume11
Issue number3
DOIs
Publication statusPublished - 2018 Mar

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

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