Selective detection of Escherichia coli by imaging of the light intensity transmitted through an optical disk

Hideyuki Shiramizu, Chiaki Kuroda, Yoshimichi Ohki, Takayuki Shima, Xiaomin Wang, Makoto Fujimaki

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    Abstract

    We have developed an optical disk system for imaging transmitted light from Escherichia coli dispersed on an optical disk. When E. coli was stained using Bismarck brown, the transmittance was found to decrease in images obtained at λ = 405 nm. The results indicate that transmittance imaging is suitable for finding the difference in light intensity between stained and unstained E. coli, whereas the reflectance images were scarcely changed by staining. Therefore, E. coli can be selectively discriminated from abiotic contaminants using transmittance imaging.

    Original languageEnglish
    Article number037001
    JournalApplied Physics Express
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 2018 Mar 1

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    ASJC Scopus subject areas

    • Engineering(all)
    • Physics and Astronomy(all)

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