Selective determination of microbial cells by graphite electrode modified with adsorbed 4,4′-bipyridine

Tadashi Matsunaga, Yoichi Namba

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The concentrations of microbial cells in suspensions can be measured with a graphite electrode modifed with adsorbed 4,4′-bipyridine, by using cyclic volummetry or differential pulse volummetry. The relationship between the peak current and the cell concentration for Saccharomyces cerevisiae is linear over the range 0.03 × 108-2.0 × 108 cells ml-1. Yeast, gram-positive bacteria and gram-negative bacteria gave different peak potentials, which may be useful for classification purposes. Electron transfer between the microbial cells and the 4,4′-bipyridine-modified electrode is mediated by coenzyme A in the cell wall.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalAnalytica Chimica Acta
Volume159
Issue numberC
DOIs
Publication statusPublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Biochemistry
  • Spectroscopy

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