Selective enrichment of two different types of Nitrospira-like nitrite-oxidizing bacteria from a wastewater treatment plant

Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Nitrification is an important step in nitrogen removal in biological wastewater treatment processes. Recently, Nitrospira have been recognized as the numerically dominant nitrite-oxidizing bacterial genus primarily responsible for the second step of aerobic nitrification; however, Nitrospira usually resist cultivation under laboratory conditions and only one species enriched from activated sludge has been described. In this study, a novel enrichment method for Nitrospira was successfully developed using continuous feeding bioreactors. By controlling nitrite concentrations strictly in the bioreactor at low levels below 10 mg-N L-1, coexisting members of sublineages I and II of the genus Nitrospira were enriched selectively. The maximum ratios of sublineages I and II to total microbial cells achieved 88.3% and 53.8%, respectively. This enrichment method is potentially applicable to other uncultured Nitrospira.

Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalMicrobes and Environments
Volume28
Issue number2
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Continuous feeding bioreactor
  • Nitrification
  • Nitrospira
  • Uncultured bacteria

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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