Selective production of glutamate by an immobilized marine blue-green alga, Synechococcus sp.

Tadashi Matsunaga, Noriyuki Nakamura, Naoko Tsuzaki, Hiroyuki Takeda

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Abstract

Among 200 strains of marine bluegreen algae isolated from the coastal areas of Japan, the marine blue-green alga Synechococcus sp. NKBG 040607 excreted glutamate at the highest rate, 82.6% of total amino acids production being glutamate. Synechococcus sp. NKBG 40607 was immobilized in calcium alginate gel. Glutamate production by immobilized cells was double that of native cells. Maximal glutamate production (25 μg/cm3 gel per day) of the immobilized cells was observed under a light intensity of 144 μEinstein/m2 per second at a cell concentration of 7.5 mg dry cells/cm3 gel. Immobilized cells of Synechococcus sp. can use nitrate as a nitrogen source. Immobilized marine Synechococcus sp. produced 0265 mg/cm3 gel of glutamate for 7 days in the presence of chloramphenicol.

Original languageEnglish
Pages (from-to)373-376
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume28
Issue number4-5
DOIs
Publication statusPublished - 1988 Jun
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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