Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Takeo Miyake, Takashi Tanii, Koichi Kato, Tamotsu Zako, Takashi Funatsu, Iwao Ohdomari

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

Original languageEnglish
Article number305304
JournalNanotechnology
Volume18
Issue number30
DOIs
Publication statusPublished - 2007 Aug 8

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Self assembled monolayers
Proteins
Adsorption
Molecules
Streptavidin

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template. / Miyake, Takeo; Tanii, Takashi; Kato, Koichi; Zako, Tamotsu; Funatsu, Takashi; Ohdomari, Iwao.

In: Nanotechnology, Vol. 18, No. 30, 305304, 08.08.2007.

Research output: Contribution to journalArticle

Miyake, Takeo ; Tanii, Takashi ; Kato, Koichi ; Zako, Tamotsu ; Funatsu, Takashi ; Ohdomari, Iwao. / Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template. In: Nanotechnology. 2007 ; Vol. 18, No. 30.
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