Self-assembly of double-stranded DNA molecules at nanomolar concentrations

Shotaro Inoue, Shigeru Sugiyama, Andrew A. Travers, Takashi Ohyama

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    Major in Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, Tokyo 169-8050, Japan Some proteins have the property of self-assembly, known to be an important mechanism in constructing supramolecular architectures for cellular functions. However, as yet, the ability of double-stranded (ds) DNA molecules to self-assemble has not been established. Here we report that dsDNA molecules also have a property of self-assembly in aqueous solutions containing physiological concentrations of Mg2+. We show that DNA molecules preferentially interact with molecules with an identical sequence and length even in a solution composed of heterogeneous DNA species. Curved DNA and DNA with an unusual conformation and property also exhibit this phenomenon, indicating that it is not specific to usual B-form DNA. Atomic force microscopy (AFM) directly reveals the assembled DNA molecules formed at concentrations of 10 nM but rarely at 1 nM. The self-assembly is concentration-dependent. We suggest that the attractive force causing DNA self-assembly may function in biological processes such as folding of repetitive DNA, recombination between homologous sequences, and synapsis in meiosis.

    Original languageEnglish
    Pages (from-to)164-171
    Number of pages8
    JournalBiochemistry
    Volume46
    Issue number1
    DOIs
    Publication statusPublished - 2007 Jan 9

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    Self assembly
    Molecules
    DNA
    B-Form DNA
    Biomedical Engineering
    Chromosome Pairing
    Biological Phenomena
    Biomedical engineering
    Tokyo
    Atomic Force Microscopy
    Meiosis
    Sequence Homology
    Genetic Recombination
    Conformations
    Atomic force microscopy
    Japan
    Proteins

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Self-assembly of double-stranded DNA molecules at nanomolar concentrations. / Inoue, Shotaro; Sugiyama, Shigeru; Travers, Andrew A.; Ohyama, Takashi.

    In: Biochemistry, Vol. 46, No. 1, 09.01.2007, p. 164-171.

    Research output: Contribution to journalArticle

    Inoue, Shotaro ; Sugiyama, Shigeru ; Travers, Andrew A. ; Ohyama, Takashi. / Self-assembly of double-stranded DNA molecules at nanomolar concentrations. In: Biochemistry. 2007 ; Vol. 46, No. 1. pp. 164-171.
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