Self-assembly of double-stranded DNA molecules at nanomolar concentrations

Shotaro Inoue, Shigeru Sugiyama, Andrew A. Travers, Takashi Ohyama

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Major in Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, 1-6-1 Nishi-Waseda, Shinjuku-ku, Tokyo 169-8050, Japan Some proteins have the property of self-assembly, known to be an important mechanism in constructing supramolecular architectures for cellular functions. However, as yet, the ability of double-stranded (ds) DNA molecules to self-assemble has not been established. Here we report that dsDNA molecules also have a property of self-assembly in aqueous solutions containing physiological concentrations of Mg2+. We show that DNA molecules preferentially interact with molecules with an identical sequence and length even in a solution composed of heterogeneous DNA species. Curved DNA and DNA with an unusual conformation and property also exhibit this phenomenon, indicating that it is not specific to usual B-form DNA. Atomic force microscopy (AFM) directly reveals the assembled DNA molecules formed at concentrations of 10 nM but rarely at 1 nM. The self-assembly is concentration-dependent. We suggest that the attractive force causing DNA self-assembly may function in biological processes such as folding of repetitive DNA, recombination between homologous sequences, and synapsis in meiosis.

Original languageEnglish
Pages (from-to)164-171
Number of pages8
JournalBiochemistry
Volume46
Issue number1
DOIs
Publication statusPublished - 2007 Jan 9

ASJC Scopus subject areas

  • Biochemistry

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