Self-assembly of quantum dots and carbon nanotubes for ultrasensitive DNA and antigen detection

Daxiang Cui, Bifeng Pan, Hong Zhang, Feng Gao, Rina Wu, Jingping Wang, Rong He, Toru Asahi

    Research output: Contribution to journalArticle

    115 Citations (Scopus)

    Abstract

    A highly selective, ultrasensitive, fluorescence detection method for DNA and antigen based on self-assembly of multiwalled carbon nanotubes (CNTs) and CdSe quantum dots (QDs) via oligonucleotide hybridization is reported. Mercaptoalkyloligonucleotide molecules bind to the quantum dots, while amineoalkyloligonucleotides bind to CNTs with -COC1 surface groups. QDs and CNTs further assemble into nanohybrids through DNA hybridization in the presence of target complementary oligonucleotides. The method is achieved with good repeatability with the detection limit of 0.2 pM DNA molecules and 0.01 nM antigen molecules. This novel detection system can also be used for multicomponent detection and antigen- antibody immunoreaction. The novel system has great potential in applications such as ultrasensitive pathogen DNA or antigen or antibody detection, molecular imaging, and photoelectrical biosensors.

    Original languageEnglish
    Pages (from-to)7996-8001
    Number of pages6
    JournalAnalytical Chemistry
    Volume80
    Issue number21
    DOIs
    Publication statusPublished - 2008 Nov 1

    Fingerprint

    Carbon Nanotubes
    Self assembly
    Semiconductor quantum dots
    Antigens
    DNA
    Oligonucleotides
    Molecules
    Molecular imaging
    Antibodies
    Multiwalled carbon nanotubes (MWCN)
    Pathogens
    Biosensors
    Fluorescence

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Self-assembly of quantum dots and carbon nanotubes for ultrasensitive DNA and antigen detection. / Cui, Daxiang; Pan, Bifeng; Zhang, Hong; Gao, Feng; Wu, Rina; Wang, Jingping; He, Rong; Asahi, Toru.

    In: Analytical Chemistry, Vol. 80, No. 21, 01.11.2008, p. 7996-8001.

    Research output: Contribution to journalArticle

    Cui, Daxiang ; Pan, Bifeng ; Zhang, Hong ; Gao, Feng ; Wu, Rina ; Wang, Jingping ; He, Rong ; Asahi, Toru. / Self-assembly of quantum dots and carbon nanotubes for ultrasensitive DNA and antigen detection. In: Analytical Chemistry. 2008 ; Vol. 80, No. 21. pp. 7996-8001.
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    AU - Wu, Rina

    AU - Wang, Jingping

    AU - He, Rong

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