Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals

Ferdinandus; Satoshi Arai; Shin'Ichi Ishiwata; Madoka Suzuki; Hirotaka Sato

doi:10.1109/TRANSDUCERS.2015.7181404

Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals

Ferdinandus, Satoshi Arai, Shin'Ichi Ishiwata, Madoka Suzuki, Hirotaka Sato^*

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

We have developed a fluorescent nanoparticle thermometer capable of mapping out the temperature distributions in small animals with higher spatial resolution than conventional infrared thermography. To acquire the fluorescence intensity change solely owing to the temperature shift, we encapsulated both the thermo-sensitive dye and the thermo-insensitive (reference) dye into the nanoparticles. The nanoparticle thermometer exhibited a significantly high thermo-sensitivity of 3.6 %/°C, and could be orally dosed into fruit fly larvae so as to stably measure the temperature distributions in the animals. This thermal-microscopy system could provide a powerful experimental paradigm to illuminate physiological functions and activities in small living organisms.

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	2228-2231
Number of pages	4
ISBN (Print)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181404
Publication status	Published - 2015 Aug 5
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: 2015 Jun 21 → 2015 Jun 25

Other

Other	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	15/6/21 → 15/6/25

Keywords

fluorescence ratiometry
fluorescent nanoparticles
Thermal microscopy
thermography
thermometer nanoparticles

ASJC Scopus subject areas

Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2015.7181404

Cite this

Ferdinandus, Arai, S., Ishiwata, SI., Suzuki, M., & Sato, H. (2015). Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 2228-2231). [7181404] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181404

Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals. / Ferdinandus; Arai, Satoshi; Ishiwata, Shin'Ichi et al.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 2228-2231 7181404.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ferdinandus, Arai, S, Ishiwata, SI, Suzuki, M & Sato, H 2015, Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181404, Institute of Electrical and Electronics Engineers Inc., pp. 2228-2231, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 15/6/21. https://doi.org/10.1109/TRANSDUCERS.2015.7181404

Ferdinandus, Arai S, Ishiwata SI, Suzuki M, Sato H. Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 2228-2231. 7181404 doi: 10.1109/TRANSDUCERS.2015.7181404

Ferdinandus ; Arai, Satoshi ; Ishiwata, Shin'Ichi et al. / Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 2228-2231

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abstract = "We have developed a fluorescent nanoparticle thermometer capable of mapping out the temperature distributions in small animals with higher spatial resolution than conventional infrared thermography. To acquire the fluorescence intensity change solely owing to the temperature shift, we encapsulated both the thermo-sensitive dye and the thermo-insensitive (reference) dye into the nanoparticles. The nanoparticle thermometer exhibited a significantly high thermo-sensitivity of 3.6 %/°C, and could be orally dosed into fruit fly larvae so as to stably measure the temperature distributions in the animals. This thermal-microscopy system could provide a powerful experimental paradigm to illuminate physiological functions and activities in small living organisms.",

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author = "Ferdinandus and Satoshi Arai and Shin'Ichi Ishiwata and Madoka Suzuki and Hirotaka Sato",

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AU - Sato, Hirotaka

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N2 - We have developed a fluorescent nanoparticle thermometer capable of mapping out the temperature distributions in small animals with higher spatial resolution than conventional infrared thermography. To acquire the fluorescence intensity change solely owing to the temperature shift, we encapsulated both the thermo-sensitive dye and the thermo-insensitive (reference) dye into the nanoparticles. The nanoparticle thermometer exhibited a significantly high thermo-sensitivity of 3.6 %/°C, and could be orally dosed into fruit fly larvae so as to stably measure the temperature distributions in the animals. This thermal-microscopy system could provide a powerful experimental paradigm to illuminate physiological functions and activities in small living organisms.

AB - We have developed a fluorescent nanoparticle thermometer capable of mapping out the temperature distributions in small animals with higher spatial resolution than conventional infrared thermography. To acquire the fluorescence intensity change solely owing to the temperature shift, we encapsulated both the thermo-sensitive dye and the thermo-insensitive (reference) dye into the nanoparticles. The nanoparticle thermometer exhibited a significantly high thermo-sensitivity of 3.6 %/°C, and could be orally dosed into fruit fly larvae so as to stably measure the temperature distributions in the animals. This thermal-microscopy system could provide a powerful experimental paradigm to illuminate physiological functions and activities in small living organisms.

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KW - fluorescent nanoparticles

KW - Thermal microscopy

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Self-calibrated fluorescent thermometer nanoparticles enable in vivo micro thermography in milimeter scale living animals

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