Self-regulating enzyme-nanotube ensemble films and their application as flexible electrodes for biofuel cells

Takeo Miyake, Syuhei Yoshino, Takeo Yamada, Kenji Hata, Matsuhiko Nishizawa

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Nanostructured carbons have been widely used for fabricating enzyme-modified electrodes due to their large specific surface area. However, because they are random aggregates of particular or tubular nanocarbons, the postmodification of enzymes to their intrananospace is generally hard to control. Here, we describe a free-standing film of carbon nanotube forest (CNTF) that can form a hybrid ensemble with enzymes through liquid-induced shrinkage. This provides in situ regulation of its intrananospace (inter-CNT pitch) to the size of enzymes and eventually serves as a highly active electrode. The CNTF ensemble with fructose dehydrogenase (FDH) showed the oxidation current density of 16 mA cm-2 in stirred 200 mM fructose solution. The power density of a biofuel cell using the FDH-CNTF anode and the Laccase-CNTF cathode reached 1.8 mW cm-2 (at 0.45 V) in the stirred oxygenic fructose solution, more than 80% of which could be maintained after continuous operation for 24 h. Application of the free-standing, flexible character of the enzyme-CNTF ensemble electrodes is demonstrated via their use in the patch or wound form.

Original languageEnglish
Pages (from-to)5129-5134
Number of pages6
JournalJournal of the American Chemical Society
Volume133
Issue number13
DOIs
Publication statusPublished - 2011 Apr 6
Externally publishedYes

Fingerprint

Bioelectric Energy Sources
Biological fuel cells
Nanotubes
Carbon Nanotubes
Fructose
Carbon nanotubes
Electrodes
Enzymes
Oxidoreductases
Laccase
Biosensing Techniques
Specific surface area
Anodes
Cathodes
Current density
Carbon
Forests
Oxidation
Wounds and Injuries
Liquids

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Self-regulating enzyme-nanotube ensemble films and their application as flexible electrodes for biofuel cells. / Miyake, Takeo; Yoshino, Syuhei; Yamada, Takeo; Hata, Kenji; Nishizawa, Matsuhiko.

In: Journal of the American Chemical Society, Vol. 133, No. 13, 06.04.2011, p. 5129-5134.

Research output: Contribution to journalArticle

Miyake, Takeo ; Yoshino, Syuhei ; Yamada, Takeo ; Hata, Kenji ; Nishizawa, Matsuhiko. / Self-regulating enzyme-nanotube ensemble films and their application as flexible electrodes for biofuel cells. In: Journal of the American Chemical Society. 2011 ; Vol. 133, No. 13. pp. 5129-5134.
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