Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Takashi Ebisawa*, Kahori Numazawa, Hiroko Shimada, Hiroyuki Izutsu, Tsukasa Sasaki, Nobumasa Kato, Katsushi Tokunaga, Akio Mori, Ken ichi Honma, Sato Honma, Shigenobu Shibata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalNeuroscience Research
Volume66
Issue number2
DOIs
Publication statusPublished - 2010 Feb

Keywords

  • Clock genes
  • Human biological clock
  • Human circadian rhythm
  • Luciferase
  • Mononuclear cells
  • Self-sustained circadian rhythm

ASJC Scopus subject areas

  • Neuroscience(all)

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