Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Takashi Ebisawa, Kahori Numazawa, Hiroko Shimada, Hiroyuki Izutsu, Tsukasa Sasaki, Nobumasa Kato, Katsushi Tokunaga, Akio Mori, Ken ichi Honma, Sato Honma, Shigenobu Shibata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalNeuroscience Research
Volume66
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1

Keywords

  • Clock genes
  • Human biological clock
  • Human circadian rhythm
  • Luciferase
  • Mononuclear cells
  • Self-sustained circadian rhythm

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood'. Together they form a unique fingerprint.

  • Cite this

    Ebisawa, T., Numazawa, K., Shimada, H., Izutsu, H., Sasaki, T., Kato, N., Tokunaga, K., Mori, A., Honma, K. I., Honma, S., & Shibata, S. (2010). Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood. Neuroscience Research, 66(2), 223-227. https://doi.org/10.1016/j.neures.2009.10.007