Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood

Takashi Ebisawa, Kahori Numazawa, Hiroko Shimada, Hiroyuki Izutsu, Tsukasa Sasaki, Nobumasa Kato, Katsushi Tokunaga, Akio Mori, Ken ichi Honma, Sato Honma, Shigenobu Shibata

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    Abstract

    Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

    Original languageEnglish
    Pages (from-to)223-227
    Number of pages5
    JournalNeuroscience Research
    Volume66
    Issue number2
    DOIs
    Publication statusPublished - 2010 Feb

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    Keywords

    • Clock genes
    • Human biological clock
    • Human circadian rhythm
    • Luciferase
    • Mononuclear cells
    • Self-sustained circadian rhythm

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Ebisawa, T., Numazawa, K., Shimada, H., Izutsu, H., Sasaki, T., Kato, N., Tokunaga, K., Mori, A., Honma, K. I., Honma, S., & Shibata, S. (2010). Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood. Neuroscience Research, 66(2), 223-227. https://doi.org/10.1016/j.neures.2009.10.007