Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones

Douglas Simon Campbell, Aoife G. Regan, Juanita S. Lopez, David Tannahill, William A. Harris, Christine E. Holt

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

The semaphorin receptor, neuropilin-1 (NP-1), was first identified in Xenopus as the A5 antigen and is expressed abundantly in developing retinal ganglion cells (RGCs). Here we show that growth cones acquire responsiveness to semaphorin 3A (Sema 3A) with age and that the onset of responsiveness correlates with the appearance of NP-1 immunoreactivity. Growth cones from "old" (stage 35/36) retinal explants collapse rapidly (5-10 min) in response to Sema 3A and turn away from a gradient of Sema 3A, whereas "young" growth cones (stage 24) are insensitive to Sema 3A. Moreover, transfection of full-length NP-1 into young neurons confers premature Sema 3A sensitivity. When young neurons are aged in culture they develop Sema 3A sensitivity in parallel with those in vivo, suggesting that an intrinsic mechanism of NP-1 regulation mediates this age-dependent change, Sema 3A-induced collapse is transient, and after recovery ∼30% of growth cones extend new branches within 1 hr, implicating Sema 3A as a branching factor. Pharmacological inhibitors were used to investigate whether these three Sema 3A-induced behaviors (collapse, turning, and branching) use distinct second messenger signaling pathways. All three behaviors were found to be mediated via cGMP. In situ hybridization shows that Sema 3A is expressed in the tectum and at the anterior boundary of the optic tract where axons bend caudally, suggesting that Sema 3A/NP-1 interactions play a role in guiding axons in the optic tract and in stimulating terminal branching in the tectum.

Original languageEnglish
Pages (from-to)8538-8547
Number of pages10
JournalJournal of Neuroscience
Volume21
Issue number21
Publication statusPublished - 2001 Nov 1
Externally publishedYes

Fingerprint

Semaphorin-3A
Retinal Cone Photoreceptor Cells
Growth Cones
Xenopus
Neuropilin-1
Axons
Semaphorins
Neurons
Retinal Ganglion Cells
Second Messenger Systems
Age of Onset
In Situ Hybridization
Transfection

Keywords

  • Axon guidance
  • Growth cone collapse
  • Neuropilin-1
  • Retinotectal projection
  • Sema 3A
  • Visual system development
  • Xenopus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Campbell, D. S., Regan, A. G., Lopez, J. S., Tannahill, D., Harris, W. A., & Holt, C. E. (2001). Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. Journal of Neuroscience, 21(21), 8538-8547.

Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. / Campbell, Douglas Simon; Regan, Aoife G.; Lopez, Juanita S.; Tannahill, David; Harris, William A.; Holt, Christine E.

In: Journal of Neuroscience, Vol. 21, No. 21, 01.11.2001, p. 8538-8547.

Research output: Contribution to journalArticle

Campbell, DS, Regan, AG, Lopez, JS, Tannahill, D, Harris, WA & Holt, CE 2001, 'Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones', Journal of Neuroscience, vol. 21, no. 21, pp. 8538-8547.
Campbell DS, Regan AG, Lopez JS, Tannahill D, Harris WA, Holt CE. Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. Journal of Neuroscience. 2001 Nov 1;21(21):8538-8547.
Campbell, Douglas Simon ; Regan, Aoife G. ; Lopez, Juanita S. ; Tannahill, David ; Harris, William A. ; Holt, Christine E. / Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 21. pp. 8538-8547.
@article{d66a82c81b954e36895e2af9ca51f709,
title = "Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones",
abstract = "The semaphorin receptor, neuropilin-1 (NP-1), was first identified in Xenopus as the A5 antigen and is expressed abundantly in developing retinal ganglion cells (RGCs). Here we show that growth cones acquire responsiveness to semaphorin 3A (Sema 3A) with age and that the onset of responsiveness correlates with the appearance of NP-1 immunoreactivity. Growth cones from {"}old{"} (stage 35/36) retinal explants collapse rapidly (5-10 min) in response to Sema 3A and turn away from a gradient of Sema 3A, whereas {"}young{"} growth cones (stage 24) are insensitive to Sema 3A. Moreover, transfection of full-length NP-1 into young neurons confers premature Sema 3A sensitivity. When young neurons are aged in culture they develop Sema 3A sensitivity in parallel with those in vivo, suggesting that an intrinsic mechanism of NP-1 regulation mediates this age-dependent change, Sema 3A-induced collapse is transient, and after recovery ∼30{\%} of growth cones extend new branches within 1 hr, implicating Sema 3A as a branching factor. Pharmacological inhibitors were used to investigate whether these three Sema 3A-induced behaviors (collapse, turning, and branching) use distinct second messenger signaling pathways. All three behaviors were found to be mediated via cGMP. In situ hybridization shows that Sema 3A is expressed in the tectum and at the anterior boundary of the optic tract where axons bend caudally, suggesting that Sema 3A/NP-1 interactions play a role in guiding axons in the optic tract and in stimulating terminal branching in the tectum.",
keywords = "Axon guidance, Growth cone collapse, Neuropilin-1, Retinotectal projection, Sema 3A, Visual system development, Xenopus",
author = "Campbell, {Douglas Simon} and Regan, {Aoife G.} and Lopez, {Juanita S.} and David Tannahill and Harris, {William A.} and Holt, {Christine E.}",
year = "2001",
month = "11",
day = "1",
language = "English",
volume = "21",
pages = "8538--8547",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "21",

}

TY - JOUR

T1 - Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones

AU - Campbell, Douglas Simon

AU - Regan, Aoife G.

AU - Lopez, Juanita S.

AU - Tannahill, David

AU - Harris, William A.

AU - Holt, Christine E.

PY - 2001/11/1

Y1 - 2001/11/1

N2 - The semaphorin receptor, neuropilin-1 (NP-1), was first identified in Xenopus as the A5 antigen and is expressed abundantly in developing retinal ganglion cells (RGCs). Here we show that growth cones acquire responsiveness to semaphorin 3A (Sema 3A) with age and that the onset of responsiveness correlates with the appearance of NP-1 immunoreactivity. Growth cones from "old" (stage 35/36) retinal explants collapse rapidly (5-10 min) in response to Sema 3A and turn away from a gradient of Sema 3A, whereas "young" growth cones (stage 24) are insensitive to Sema 3A. Moreover, transfection of full-length NP-1 into young neurons confers premature Sema 3A sensitivity. When young neurons are aged in culture they develop Sema 3A sensitivity in parallel with those in vivo, suggesting that an intrinsic mechanism of NP-1 regulation mediates this age-dependent change, Sema 3A-induced collapse is transient, and after recovery ∼30% of growth cones extend new branches within 1 hr, implicating Sema 3A as a branching factor. Pharmacological inhibitors were used to investigate whether these three Sema 3A-induced behaviors (collapse, turning, and branching) use distinct second messenger signaling pathways. All three behaviors were found to be mediated via cGMP. In situ hybridization shows that Sema 3A is expressed in the tectum and at the anterior boundary of the optic tract where axons bend caudally, suggesting that Sema 3A/NP-1 interactions play a role in guiding axons in the optic tract and in stimulating terminal branching in the tectum.

AB - The semaphorin receptor, neuropilin-1 (NP-1), was first identified in Xenopus as the A5 antigen and is expressed abundantly in developing retinal ganglion cells (RGCs). Here we show that growth cones acquire responsiveness to semaphorin 3A (Sema 3A) with age and that the onset of responsiveness correlates with the appearance of NP-1 immunoreactivity. Growth cones from "old" (stage 35/36) retinal explants collapse rapidly (5-10 min) in response to Sema 3A and turn away from a gradient of Sema 3A, whereas "young" growth cones (stage 24) are insensitive to Sema 3A. Moreover, transfection of full-length NP-1 into young neurons confers premature Sema 3A sensitivity. When young neurons are aged in culture they develop Sema 3A sensitivity in parallel with those in vivo, suggesting that an intrinsic mechanism of NP-1 regulation mediates this age-dependent change, Sema 3A-induced collapse is transient, and after recovery ∼30% of growth cones extend new branches within 1 hr, implicating Sema 3A as a branching factor. Pharmacological inhibitors were used to investigate whether these three Sema 3A-induced behaviors (collapse, turning, and branching) use distinct second messenger signaling pathways. All three behaviors were found to be mediated via cGMP. In situ hybridization shows that Sema 3A is expressed in the tectum and at the anterior boundary of the optic tract where axons bend caudally, suggesting that Sema 3A/NP-1 interactions play a role in guiding axons in the optic tract and in stimulating terminal branching in the tectum.

KW - Axon guidance

KW - Growth cone collapse

KW - Neuropilin-1

KW - Retinotectal projection

KW - Sema 3A

KW - Visual system development

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=0035503546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035503546&partnerID=8YFLogxK

M3 - Article

C2 - 11606642

AN - SCOPUS:0035503546

VL - 21

SP - 8538

EP - 8547

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 21

ER -