Semaphorin3A signaling mediated by fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32

Yutaka Uchida, Toshio Ohshima, Naoya Yamashita, Miyuki Ogawara, Yukio Sasaki, Fumio Nakamura, Yoshio Goshima

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    Collapsin response mediator protein 2 (CRMP2) is an intracellular protein that mediates signaling of Semaphorin3A (Sema3A), a repulsive axon guidance molecule. Fyn, a Src-type tyrosine kinase, is involved in the Sema3A signaling. However, the relationship betweenCRMP2and Fyn in this signaling pathway is still unknown. In our research, we demonstrated that Fyn phosphorylated CRMP2 at Tyr32 residues in HEK293T cells. Immunohistochemical analysis using a phospho-specific antibody at Tyr32 of CRMP showed that Tyr32-phosphorylated CRMP was abundant in the nervous system, including dorsal root ganglion neurons, the molecular and Purkinje cell layer of adult cerebellum, and hippocampal fimbria. Overexpression of a nonphosphorylated mutant (Tyr32 to Phe32) of CRMP2 in dorsal root ganglion neurons interfered with Sema3A-induced growth cone collapse response. These results suggest that Fyn-dependent phosphorylation of CRMP2 at Tyr32 is involved in Sema3A signaling.

    Original languageEnglish
    Pages (from-to)27393-27401
    Number of pages9
    JournalJournal of Biological Chemistry
    Volume284
    Issue number40
    DOIs
    Publication statusPublished - 2009 Oct 2

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    Phosphorylation
    Tyrosine
    Spinal Ganglia
    Neurons
    Intracellular Signaling Peptides and Proteins
    Phospho-Specific Antibodies
    Growth Cones
    src-Family Kinases
    Purkinje Cells
    Neurology
    Cerebellum
    Nervous System
    Cones
    Cells
    Molecules
    collapsin response mediator protein-2
    2-tyrosine
    Research

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology
    • Medicine(all)

    Cite this

    Semaphorin3A signaling mediated by fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32. / Uchida, Yutaka; Ohshima, Toshio; Yamashita, Naoya; Ogawara, Miyuki; Sasaki, Yukio; Nakamura, Fumio; Goshima, Yoshio.

    In: Journal of Biological Chemistry, Vol. 284, No. 40, 02.10.2009, p. 27393-27401.

    Research output: Contribution to journalArticle

    Uchida, Yutaka ; Ohshima, Toshio ; Yamashita, Naoya ; Ogawara, Miyuki ; Sasaki, Yukio ; Nakamura, Fumio ; Goshima, Yoshio. / Semaphorin3A signaling mediated by fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 40. pp. 27393-27401.
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    abstract = "Collapsin response mediator protein 2 (CRMP2) is an intracellular protein that mediates signaling of Semaphorin3A (Sema3A), a repulsive axon guidance molecule. Fyn, a Src-type tyrosine kinase, is involved in the Sema3A signaling. However, the relationship betweenCRMP2and Fyn in this signaling pathway is still unknown. In our research, we demonstrated that Fyn phosphorylated CRMP2 at Tyr32 residues in HEK293T cells. Immunohistochemical analysis using a phospho-specific antibody at Tyr32 of CRMP showed that Tyr32-phosphorylated CRMP was abundant in the nervous system, including dorsal root ganglion neurons, the molecular and Purkinje cell layer of adult cerebellum, and hippocampal fimbria. Overexpression of a nonphosphorylated mutant (Tyr32 to Phe32) of CRMP2 in dorsal root ganglion neurons interfered with Sema3A-induced growth cone collapse response. These results suggest that Fyn-dependent phosphorylation of CRMP2 at Tyr32 is involved in Sema3A signaling.",
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    AU - Yamashita, Naoya

    AU - Ogawara, Miyuki

    AU - Sasaki, Yukio

    AU - Nakamura, Fumio

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