Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer

H. Okada, A. Iguchi, R. Iizuka, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, T. Funatsu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1259-1261
Number of pages3
ISBN (Print)9780979806476
Publication statusPublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: 2014 Oct 262014 Oct 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period14/10/2614/10/30

Fingerprint

Gelation
Sorting
Fluorescence
Polymers
Microfluidics
Chromosomes
Demonstrations

Keywords

  • Droplet sorting
  • On-chip sorting
  • Proteomics
  • Thermoreversible gelation polymer

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Okada, H., Iguchi, A., Iizuka, R., Yoon, D., Sekiguchi, T., Shoji, S., & Funatsu, T. (2014). Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1259-1261). Chemical and Biological Microsystems Society.

Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer. / Okada, H.; Iguchi, A.; Iizuka, R.; Yoon, Donghyun; Sekiguchi, Tetsushi; Shoji, Shuichi; Funatsu, T.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1259-1261.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okada, H, Iguchi, A, Iizuka, R, Yoon, D, Sekiguchi, T, Shoji, S & Funatsu, T 2014, Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 1259-1261, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.
Okada H, Iguchi A, Iizuka R, Yoon D, Sekiguchi T, Shoji S et al. Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1259-1261
Okada, H. ; Iguchi, A. ; Iizuka, R. ; Yoon, Donghyun ; Sekiguchi, Tetsushi ; Shoji, Shuichi ; Funatsu, T. / Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1259-1261
@inproceedings{6480e9565b1548648b6206eafb725dac,
title = "Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer",
abstract = "We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.",
keywords = "Droplet sorting, On-chip sorting, Proteomics, Thermoreversible gelation polymer",
author = "H. Okada and A. Iguchi and R. Iizuka and Donghyun Yoon and Tetsushi Sekiguchi and Shuichi Shoji and T. Funatsu",
year = "2014",
language = "English",
isbn = "9780979806476",
pages = "1259--1261",
booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",
publisher = "Chemical and Biological Microsystems Society",

}

TY - GEN

T1 - Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer

AU - Okada, H.

AU - Iguchi, A.

AU - Iizuka, R.

AU - Yoon, Donghyun

AU - Sekiguchi, Tetsushi

AU - Shoji, Shuichi

AU - Funatsu, T.

PY - 2014

Y1 - 2014

N2 - We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.

AB - We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.

KW - Droplet sorting

KW - On-chip sorting

KW - Proteomics

KW - Thermoreversible gelation polymer

UR - http://www.scopus.com/inward/record.url?scp=84941687359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941687359&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9780979806476

SP - 1259

EP - 1261

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

ER -