Sequence analysis of the putative regulatory region of rat α2-macroglobulin gene

Tsuchiya Yoshihiro; Masahira Hattori; Hayashida Kazuhiro; Ishibashi Hiromi; Okubo Hideo; Sakaki Yoshiyuki

doi:10.1016/0378-1119(87)90178-8

Sequence analysis of the putative regulatory region of rat α₂-macroglobulin gene

Tsuchiya Yoshihiro, Masahira Hattori, Hayashida Kazuhiro, Ishibashi Hiromi, Okubo Hideo, Sakaki Yoshiyuki

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Rat α₂-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	Gene
Volume	57
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(87)90178-8
Publication status	Published - 1987
Externally published	Yes

Keywords

acute phase protein
capping site
genomic DNA library
glucocorticoid
human gene
Recombinant DNA

ASJC Scopus subject areas

Genetics

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title = "Sequence analysis of the putative regulatory region of rat α2-macroglobulin gene",

abstract = "Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.",

keywords = "acute phase protein, capping site, genomic DNA library, glucocorticoid, human gene, Recombinant DNA",

author = "Tsuchiya Yoshihiro and Masahira Hattori and Hayashida Kazuhiro and Ishibashi Hiromi and Okubo Hideo and Sakaki Yoshiyuki",

year = "1987",

doi = "10.1016/0378-1119(87)90178-8",

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T1 - Sequence analysis of the putative regulatory region of rat α2-macroglobulin gene

AU - Yoshihiro, Tsuchiya

AU - Hattori, Masahira

AU - Kazuhiro, Hayashida

AU - Hiromi, Ishibashi

AU - Hideo, Okubo

AU - Yoshiyuki, Sakaki

PY - 1987

Y1 - 1987

N2 - Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.

AB - Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.

KW - acute phase protein

KW - capping site

KW - genomic DNA library

KW - glucocorticoid

KW - human gene

KW - Recombinant DNA

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