Abstract
Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.
| Original language | English |
|---|---|
| Pages (from-to) | 73-80 |
| Number of pages | 8 |
| Journal | Gene |
| Volume | 57 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1987 |
| Externally published | Yes |
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Keywords
- acute phase protein
- capping site
- genomic DNA library
- glucocorticoid
- human gene
- Recombinant DNA
ASJC Scopus subject areas
- Genetics
Cite this
Sequence analysis of the putative regulatory region of rat α2-macroglobulin gene. / Yoshihiro, Tsuchiya; Hattori, Masahira; Kazuhiro, Hayashida; Hiromi, Ishibashi; Hideo, Okubo; Yoshiyuki, Sakaki.
In: Gene, Vol. 57, No. 1, 1987, p. 73-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence analysis of the putative regulatory region of rat α2-macroglobulin gene
AU - Yoshihiro, Tsuchiya
AU - Hattori, Masahira
AU - Kazuhiro, Hayashida
AU - Hiromi, Ishibashi
AU - Hideo, Okubo
AU - Yoshiyuki, Sakaki
PY - 1987
Y1 - 1987
N2 - Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.
AB - Rat α2-macroglobulin (α2M) is an acute-phase reactant, concentration of which in serum increases more than 100-fold in the course of inflammation. Glucocorticoid and some protein factors such as interleukin 1 (IL-1) have been known to be involved in the regulation of this plasma protein synthesis. To understand the regulatory mechanism of α2M production at the molecular level, we isolated genomic DNA clones of rat α2M gene and characterized the promoter region of the gene by comparing the nucleotide sequence with those of other acute-phase reactant genes. Several possible regulatory signals were identified. Particularly, a sequence (T/A)T(C/G)TGGGA(A/T) was found about at 170 bp upstream from a putative capping site, which was also found in the 5'-flanking region of various acute-phase reactant genes.
KW - acute phase protein
KW - capping site
KW - genomic DNA library
KW - glucocorticoid
KW - human gene
KW - Recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=0023269921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023269921&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(87)90178-8
DO - 10.1016/0378-1119(87)90178-8
M3 - Article
C2 - 2448189
AN - SCOPUS:0023269921
VL - 57
SP - 73
EP - 80
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -