Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.

Ryuji Kawaguchi; Takayuki Nagaoka; J. Grant Burgess; Haruko Takeyama; Tadashi Matsunaga

doi:10.1006/plas.1994.1063

Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.

Ryuji Kawaguchi, Takayuki Nagaoka, J. Grant Burgess, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.

Original language	English
Pages (from-to)	245-253
Number of pages	9
Journal	Plasmid
Volume	32
Issue number	3
DOIs	https://doi.org/10.1006/plas.1994.1063
Publication status	Published - 1994 Nov
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology

Access to Document

10.1006/plas.1994.1063

Fingerprint Dive into the research topics of 'Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.'. Together they form a unique fingerprint.

Cite this

Kawaguchi, R., Nagaoka, T., Burgess, J. G., Takeyama, H., & Matsunaga, T. (1994). Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp. Plasmid, 32(3), 245-253. https://doi.org/10.1006/plas.1994.1063

@article{b3319ebbfdda422298c6b7e4398a4c50,

title = "Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.",

abstract = "We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.",

author = "Ryuji Kawaguchi and Takayuki Nagaoka and Burgess, {J. Grant} and Haruko Takeyama and Tadashi Matsunaga",

year = "1994",

month = nov,

doi = "10.1006/plas.1994.1063",

language = "English",

volume = "32",

pages = "245--253",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Sequence of a 2.6-kb cryptic plasmid from a marine cyanobacterium synechococcus sp.

AU - Kawaguchi, Ryuji

AU - Nagaoka, Takayuki

AU - Burgess, J. Grant

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

PY - 1994/11

Y1 - 1994/11

N2 - We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.

AB - We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb EcoRI fragment. This ORF encodes a putative protein which is 360 aa residues in length and is 37.8% homologous to the replication protein of plasmid pCA2.4 from Synechocystis sp. strain PCC 6803, 35.8% homologous to an ORF from the Nostoc plasmid pGL2, and 33.2% homologous to the ORF of a plasmid from Lactobacillus plantarum, pC30il. Highly conserved regions of amino acid sequence were also found between ORF-B and other bacterial plasmids.

UR - http://www.scopus.com/inward/record.url?scp=0028037358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028037358&partnerID=8YFLogxK

U2 - 10.1006/plas.1994.1063

DO - 10.1006/plas.1994.1063

M3 - Article

C2 - 7899510

AN - SCOPUS:0028037358

VL - 32

SP - 245

EP - 253

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 3

ER -