Sequential changes in genome-wide DNA methylation status during adipocyte differentiation

Hideki Sakamoto, Yasushi Kogo, Jun Ohgane, Naka Hattori, Shintaro Yagi, Satoshi Tanaka, Kunio Shiota

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

DNA methylation is an epigenetic mark on the mammalian genome. There are numerous tissue-dependent and differentially methylated regions (T-DMRs) in the unique sequences distributed throughout the genome. To determine the epigenetic changes during adipocyte differentiation, we investigated the sequential changes in DNA methylation status of 3T3-L1 cells at the growing, confluent, postconfluent and mature adipocyte cell stages. Treatment of 3T3-L1 cells with 5-aza-2′-deoxycytidine inhibited differentiation in a stage-dependent manner, supporting the idea that formation of accurate DNA methylation profile, consisting of methylated and unmethylated T-DMRs, may be involved in differentiation. Analysis by methylation-sensitive quantitative real-time PCR of the 65 known T-DMRs which contain NotI sites detected 8 methylations that changed during differentiation, and the changes in the patterns of these methylations were diverse, confirming that the differentiation process involves epigenetic alteration at the T-DMRs. Intriguingly, the dynamics of the methylation change vary depending on the T-DMRs and differentiation stages. Restriction landmark genomic scanning detected 32 novel T-DMRs, demonstrating that differentiation of 3T3-L1 cells involves genome-wide epigenetic changes by temporal methylation/demethylation, in addition to maintenance of a static methylated/demethylated state, and both depend on differentiation stage.

Original languageEnglish
Pages (from-to)360-366
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number2
DOIs
Publication statusPublished - 2008 Feb 8
Externally publishedYes

Fingerprint

DNA Methylation
Methylation
Adipocytes
Genes
Genome
Tissue
3T3-L1 Cells
Epigenomics
decitabine
Genetic Epigenesis
Real-Time Polymerase Chain Reaction
Maintenance
Scanning

Keywords

  • Adipocyte differentiation
  • DNA methylation
  • Restriction landmark genomic scanning
  • Tissue-dependent and differentially methylated regions

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sequential changes in genome-wide DNA methylation status during adipocyte differentiation. / Sakamoto, Hideki; Kogo, Yasushi; Ohgane, Jun; Hattori, Naka; Yagi, Shintaro; Tanaka, Satoshi; Shiota, Kunio.

In: Biochemical and Biophysical Research Communications, Vol. 366, No. 2, 08.02.2008, p. 360-366.

Research output: Contribution to journalArticle

Sakamoto, Hideki ; Kogo, Yasushi ; Ohgane, Jun ; Hattori, Naka ; Yagi, Shintaro ; Tanaka, Satoshi ; Shiota, Kunio. / Sequential changes in genome-wide DNA methylation status during adipocyte differentiation. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 366, No. 2. pp. 360-366.
@article{34db296141e140a4a2f8e8a5dc7d3e22,
title = "Sequential changes in genome-wide DNA methylation status during adipocyte differentiation",
abstract = "DNA methylation is an epigenetic mark on the mammalian genome. There are numerous tissue-dependent and differentially methylated regions (T-DMRs) in the unique sequences distributed throughout the genome. To determine the epigenetic changes during adipocyte differentiation, we investigated the sequential changes in DNA methylation status of 3T3-L1 cells at the growing, confluent, postconfluent and mature adipocyte cell stages. Treatment of 3T3-L1 cells with 5-aza-2′-deoxycytidine inhibited differentiation in a stage-dependent manner, supporting the idea that formation of accurate DNA methylation profile, consisting of methylated and unmethylated T-DMRs, may be involved in differentiation. Analysis by methylation-sensitive quantitative real-time PCR of the 65 known T-DMRs which contain NotI sites detected 8 methylations that changed during differentiation, and the changes in the patterns of these methylations were diverse, confirming that the differentiation process involves epigenetic alteration at the T-DMRs. Intriguingly, the dynamics of the methylation change vary depending on the T-DMRs and differentiation stages. Restriction landmark genomic scanning detected 32 novel T-DMRs, demonstrating that differentiation of 3T3-L1 cells involves genome-wide epigenetic changes by temporal methylation/demethylation, in addition to maintenance of a static methylated/demethylated state, and both depend on differentiation stage.",
keywords = "Adipocyte differentiation, DNA methylation, Restriction landmark genomic scanning, Tissue-dependent and differentially methylated regions",
author = "Hideki Sakamoto and Yasushi Kogo and Jun Ohgane and Naka Hattori and Shintaro Yagi and Satoshi Tanaka and Kunio Shiota",
year = "2008",
month = "2",
day = "8",
doi = "10.1016/j.bbrc.2007.11.137",
language = "English",
volume = "366",
pages = "360--366",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Sequential changes in genome-wide DNA methylation status during adipocyte differentiation

AU - Sakamoto, Hideki

AU - Kogo, Yasushi

AU - Ohgane, Jun

AU - Hattori, Naka

AU - Yagi, Shintaro

AU - Tanaka, Satoshi

AU - Shiota, Kunio

PY - 2008/2/8

Y1 - 2008/2/8

N2 - DNA methylation is an epigenetic mark on the mammalian genome. There are numerous tissue-dependent and differentially methylated regions (T-DMRs) in the unique sequences distributed throughout the genome. To determine the epigenetic changes during adipocyte differentiation, we investigated the sequential changes in DNA methylation status of 3T3-L1 cells at the growing, confluent, postconfluent and mature adipocyte cell stages. Treatment of 3T3-L1 cells with 5-aza-2′-deoxycytidine inhibited differentiation in a stage-dependent manner, supporting the idea that formation of accurate DNA methylation profile, consisting of methylated and unmethylated T-DMRs, may be involved in differentiation. Analysis by methylation-sensitive quantitative real-time PCR of the 65 known T-DMRs which contain NotI sites detected 8 methylations that changed during differentiation, and the changes in the patterns of these methylations were diverse, confirming that the differentiation process involves epigenetic alteration at the T-DMRs. Intriguingly, the dynamics of the methylation change vary depending on the T-DMRs and differentiation stages. Restriction landmark genomic scanning detected 32 novel T-DMRs, demonstrating that differentiation of 3T3-L1 cells involves genome-wide epigenetic changes by temporal methylation/demethylation, in addition to maintenance of a static methylated/demethylated state, and both depend on differentiation stage.

AB - DNA methylation is an epigenetic mark on the mammalian genome. There are numerous tissue-dependent and differentially methylated regions (T-DMRs) in the unique sequences distributed throughout the genome. To determine the epigenetic changes during adipocyte differentiation, we investigated the sequential changes in DNA methylation status of 3T3-L1 cells at the growing, confluent, postconfluent and mature adipocyte cell stages. Treatment of 3T3-L1 cells with 5-aza-2′-deoxycytidine inhibited differentiation in a stage-dependent manner, supporting the idea that formation of accurate DNA methylation profile, consisting of methylated and unmethylated T-DMRs, may be involved in differentiation. Analysis by methylation-sensitive quantitative real-time PCR of the 65 known T-DMRs which contain NotI sites detected 8 methylations that changed during differentiation, and the changes in the patterns of these methylations were diverse, confirming that the differentiation process involves epigenetic alteration at the T-DMRs. Intriguingly, the dynamics of the methylation change vary depending on the T-DMRs and differentiation stages. Restriction landmark genomic scanning detected 32 novel T-DMRs, demonstrating that differentiation of 3T3-L1 cells involves genome-wide epigenetic changes by temporal methylation/demethylation, in addition to maintenance of a static methylated/demethylated state, and both depend on differentiation stage.

KW - Adipocyte differentiation

KW - DNA methylation

KW - Restriction landmark genomic scanning

KW - Tissue-dependent and differentially methylated regions

UR - http://www.scopus.com/inward/record.url?scp=37449022722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37449022722&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.11.137

DO - 10.1016/j.bbrc.2007.11.137

M3 - Article

C2 - 18062916

AN - SCOPUS:37449022722

VL - 366

SP - 360

EP - 366

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -