Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells

Yasuhiro Okamoto, Yoshifumi Kawano, Yoichi Takaue, Tsutomu Watanabe, Takashi Kato, Akihiro Shimosaka, Yasuhiro Kuroda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In a case control study, serum levels of thrombopoietin (TPO) were determined by a sandwich ELISA in 20 patients (median age, 7 years; range, 2- 56 years) with various malignancies who received high-dose chemotherapy and a stem cell rescue operation. The patients received two different transplant modalities: (a) 12 patients received purified autologous peripheral blood CD34+ cells; and (b) 8 patients received cells in the CD34(-) fraction, which still contains many CD34+ cells. No significant differences were observed between the two groups with regard to the duration required to achieve an absolute granulocyte count of >0.5 x 109/liter, the duration of dependence on platelet transfusion, or the number of platelet transfusions. In both groups, the serum TPO levels were inversely correlated with the circulating platelet count. Multivariate analysis demonstrated that significant determinants of the serum TPO level included the circulating platelet count (standardized regression coefficient = -0.5179), transplantation with cells in the CD34(-) fraction (0.2414), solid tumor (0.1420), and the age of the patient (-0.1236; r2 = 0.3021; P < 0.0001). These results suggest that the mode of stem cell support (i.e., the presence of accessory cells in the inoculum), age, or the type of preceding chemotherapy affects serum TPO levels after transplantation.

Original languageEnglish
Pages (from-to)5037-5040
Number of pages4
JournalCancer Research
Volume57
Issue number22
Publication statusPublished - 1997 Nov 15
Externally publishedYes

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Thrombopoietin
Blood Cells
Drug Therapy
Serum
Platelet Transfusion
Platelet Count
Stem Cells
Cell Transplantation
Granulocytes
Case-Control Studies
Neoplasms
Multivariate Analysis
Transplantation
Enzyme-Linked Immunosorbent Assay
Transplants

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Okamoto, Y., Kawano, Y., Takaue, Y., Watanabe, T., Kato, T., Shimosaka, A., & Kuroda, Y. (1997). Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells. Cancer Research, 57(22), 5037-5040.

Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells. / Okamoto, Yasuhiro; Kawano, Yoshifumi; Takaue, Yoichi; Watanabe, Tsutomu; Kato, Takashi; Shimosaka, Akihiro; Kuroda, Yasuhiro.

In: Cancer Research, Vol. 57, No. 22, 15.11.1997, p. 5037-5040.

Research output: Contribution to journalArticle

Okamoto, Y, Kawano, Y, Takaue, Y, Watanabe, T, Kato, T, Shimosaka, A & Kuroda, Y 1997, 'Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells', Cancer Research, vol. 57, no. 22, pp. 5037-5040.
Okamoto, Yasuhiro ; Kawano, Yoshifumi ; Takaue, Yoichi ; Watanabe, Tsutomu ; Kato, Takashi ; Shimosaka, Akihiro ; Kuroda, Yasuhiro. / Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells. In: Cancer Research. 1997 ; Vol. 57, No. 22. pp. 5037-5040.
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