Sex determination in amphibians

Masahisa Nakamura

    Research output: Contribution to journalArticle

    88 Citations (Scopus)

    Abstract

    The heterogametic sex is male in all mammals, whereas it is female in almost all birds. By contrast, there are two heterogametic types (XX/XY and ZZ/ZW) for genetic sex determination in amphibians. Though the original heterogametic sex was female in amphibians, the two heterogametic types were probably interchangeable, suggesting that sex chromosomes evolved several times in this lineage. Indeed, the frog Rana rugosa has the XX/XY and ZZ/ZW sex-determining systems within a single species, depending on the local population in Japan. The XY and ZW geographic forms with differentiated sex chromosomes probably have a common origin as undifferentiated sex chromosomes resulted from the hybridization between the primary populations of West Japan and Kanto forms. It is clear that the sex chromosomes are still undergoing evolution in this species group. Regardless of the presence of a sex-determining gene in amphibians, the gonadal sex of some species can be changed by sex steroids. Namely, sex steroids can induce the sex reversal, with estrogens inducing the male-to-female sex reversal, whereas androgens have the opposite effect. In R. rugosa, gonadal activity of CYP19 (P450 aromatase) is correlated with the feminization of gonads. Of particular interest is that high levels of CYP19 expression are observed in indifferent gonads at time before sex determination. Increases in the expression of CYP19 in female gonads and CYP17 (P450 17α-hydroxylase/C17-20 lyase) in male gonads suggest that the former plays an important role in phenotypic female determination, whereas the latter is needed for male determination. Thus, steroids could be the key factor for sex determination in R. rugosa. In addition to the role of sex steroids in gonadal sex determination in this species, Foxl2 and Sox3 are capable of promoting CYP19 expression. Since both the genes are autosomal, another factor up-regulating CYP19 expression must be recruited. The factor, which may be located on the X or W chromosome, intervenes directly or indirectly, in the transcriptional regulation of the CYP19 gene for feminization in amphibians. A factor up-regulating CYP17 expression remains to be identified.

    Original languageEnglish
    Pages (from-to)271-282
    Number of pages12
    JournalSeminars in Cell and Developmental Biology
    Volume20
    Issue number3
    DOIs
    Publication statusPublished - 2009 May

    Fingerprint

    Amphibians
    Aromatase
    Sex Chromosomes
    Gonads
    Steroids
    Feminization
    Steroid 17-alpha-Hydroxylase
    Japan
    Sex Determination Processes
    Genes
    Ranidae
    Sex Factors
    Lyases
    Mixed Function Oxygenases
    Anura
    Population
    Androgens
    Birds
    Mammals
    Estrogens

    Keywords

    • Amphibians
    • CYP17
    • CYP19
    • ESD
    • Foxl2
    • GSD
    • Rana rugosa
    • Sex chromosome
    • Sex reversal
    • Sex steroids
    • SF1

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

    Cite this

    Sex determination in amphibians. / Nakamura, Masahisa.

    In: Seminars in Cell and Developmental Biology, Vol. 20, No. 3, 05.2009, p. 271-282.

    Research output: Contribution to journalArticle

    Nakamura, Masahisa. / Sex determination in amphibians. In: Seminars in Cell and Developmental Biology. 2009 ; Vol. 20, No. 3. pp. 271-282.
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