Sex difference in septal neurons projecting axons to midbrain central gray in rats

A combined double retrograde tracing and ER-immunohistochemical study

Shinji Tsukahara, Korehito Yamanouchi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunohistochemistry for ER-α and -β was also performed with or without combination with FG immunostaining. All animals were gonadectomized. Lordosis was observed after treatment with E2 in some animals. In the results, lordosis was rare in males, compared with females. FG-immunoreactive (ir) cells were concentrated in the intermediate LS on the right side, and its number in the females was significantly higher than that in the males. There was no sex difference in the distribution and number of ERα-ir and ERβ-ir cells in the LS. Furthermore, the number of ERs-ir cells was not influenced by E2 in either males or females. Double FG-ERβ-ir cells were less than 20% of total FG-ir cells in the LS in both males and females. These data suggest that the LS-MCG connection is sexually dimorphic but that there is no sex difference in the expression of ERs in the LS.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalEndocrinology
Volume143
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Periaqueductal Gray
Sex Characteristics
Axons
Lordosis
Neurons
Immunohistochemistry
methanesulfonate salt 2-hydroxy-4,4'-diamidinostilbene
Injections

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Sex difference in septal neurons projecting axons to midbrain central gray in rats : A combined double retrograde tracing and ER-immunohistochemical study. / Tsukahara, Shinji; Yamanouchi, Korehito.

In: Endocrinology, Vol. 143, No. 1, 2002, p. 285-294.

Research output: Contribution to journalArticle

@article{d8f5c2237e4e4f88aa9ddab46ecea173,
title = "Sex difference in septal neurons projecting axons to midbrain central gray in rats: A combined double retrograde tracing and ER-immunohistochemical study",
abstract = "Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunohistochemistry for ER-α and -β was also performed with or without combination with FG immunostaining. All animals were gonadectomized. Lordosis was observed after treatment with E2 in some animals. In the results, lordosis was rare in males, compared with females. FG-immunoreactive (ir) cells were concentrated in the intermediate LS on the right side, and its number in the females was significantly higher than that in the males. There was no sex difference in the distribution and number of ERα-ir and ERβ-ir cells in the LS. Furthermore, the number of ERs-ir cells was not influenced by E2 in either males or females. Double FG-ERβ-ir cells were less than 20{\%} of total FG-ir cells in the LS in both males and females. These data suggest that the LS-MCG connection is sexually dimorphic but that there is no sex difference in the expression of ERs in the LS.",
author = "Shinji Tsukahara and Korehito Yamanouchi",
year = "2002",
doi = "10.1210/en.143.1.285",
language = "English",
volume = "143",
pages = "285--294",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Sex difference in septal neurons projecting axons to midbrain central gray in rats

T2 - A combined double retrograde tracing and ER-immunohistochemical study

AU - Tsukahara, Shinji

AU - Yamanouchi, Korehito

PY - 2002

Y1 - 2002

N2 - Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunohistochemistry for ER-α and -β was also performed with or without combination with FG immunostaining. All animals were gonadectomized. Lordosis was observed after treatment with E2 in some animals. In the results, lordosis was rare in males, compared with females. FG-immunoreactive (ir) cells were concentrated in the intermediate LS on the right side, and its number in the females was significantly higher than that in the males. There was no sex difference in the distribution and number of ERα-ir and ERβ-ir cells in the LS. Furthermore, the number of ERs-ir cells was not influenced by E2 in either males or females. Double FG-ERβ-ir cells were less than 20% of total FG-ir cells in the LS in both males and females. These data suggest that the LS-MCG connection is sexually dimorphic but that there is no sex difference in the expression of ERs in the LS.

AB - Sex difference in the number of neurons projecting axons from the lateral septum (LS) to the midbrain central gray (MCG) that are concerned with the lordosis-inhibiting system was investigated by injection of Fluoro-Gold (FG), a retrograde tracer, into the rostral MCG on the right side in male and female rats. Immunohistochemistry for ER-α and -β was also performed with or without combination with FG immunostaining. All animals were gonadectomized. Lordosis was observed after treatment with E2 in some animals. In the results, lordosis was rare in males, compared with females. FG-immunoreactive (ir) cells were concentrated in the intermediate LS on the right side, and its number in the females was significantly higher than that in the males. There was no sex difference in the distribution and number of ERα-ir and ERβ-ir cells in the LS. Furthermore, the number of ERs-ir cells was not influenced by E2 in either males or females. Double FG-ERβ-ir cells were less than 20% of total FG-ir cells in the LS in both males and females. These data suggest that the LS-MCG connection is sexually dimorphic but that there is no sex difference in the expression of ERs in the LS.

UR - http://www.scopus.com/inward/record.url?scp=0036141055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036141055&partnerID=8YFLogxK

U2 - 10.1210/en.143.1.285

DO - 10.1210/en.143.1.285

M3 - Article

VL - 143

SP - 285

EP - 294

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 1

ER -