SFT-4/Surf4 control ER export of soluble cargo proteins and participate in ER exit site organization

Keiko Saegusa, Miyuki Sato, Nobukatsu Morooka, Taichi Hara, Ken Sato

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Lipoproteins regulate the overall lipid homeostasis in animals. However, the molecular mechanisms underlying lipoprotein trafficking remain poorly understood. Here, we show that SFT-4, a Caenorhabditis elegans homologue of the yeast Erv29p, is essential for the endoplasmic reticulum (ER) export of the yolk protein VIT-2, which is synthesized as a lipoprotein complex. SFT-4 loss strongly inhibits the ER exit of yolk proteins and certain soluble cargo proteins in intestinal cells. SFT-4 predominantly localizes at ER exit sites (ERES) and physically interacts with VIT-2 in vivo, which suggests that SFT-4 promotes the ER export of soluble proteins as a cargo receptor. Notably, Surf4, a mammalian SFT-4 homologue, physically interacts with apolipoprotein B, a very-low-density lipoprotein core protein, and its loss causes ER accumulation of apolipoprotein B in human hepatic HepG2 cells. Interestingly, loss of SFT-4 and Surf4 reduced the number of COP II-positive ERES. Thus, SFT-4 and Surf4 regulate the export of soluble proteins, including lipoproteins, from the ER and participate in ERES organization in animals.

    Original languageEnglish
    Pages (from-to)2073-2085
    Number of pages13
    JournalJournal of Cell Biology
    Volume217
    Issue number6
    DOIs
    Publication statusPublished - 2018 Jun 1

    Fingerprint

    Endoplasmic Reticulum
    Lipoproteins
    Egg Proteins
    Proteins
    Apolipoproteins B
    VLDL Lipoproteins
    Caenorhabditis elegans
    Hep G2 Cells
    Hepatocytes
    Homeostasis
    Yeasts
    Lipids

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    SFT-4/Surf4 control ER export of soluble cargo proteins and participate in ER exit site organization. / Saegusa, Keiko; Sato, Miyuki; Morooka, Nobukatsu; Hara, Taichi; Sato, Ken.

    In: Journal of Cell Biology, Vol. 217, No. 6, 01.06.2018, p. 2073-2085.

    Research output: Contribution to journalArticle

    Saegusa, Keiko ; Sato, Miyuki ; Morooka, Nobukatsu ; Hara, Taichi ; Sato, Ken. / SFT-4/Surf4 control ER export of soluble cargo proteins and participate in ER exit site organization. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 6. pp. 2073-2085.
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