Sharpea azabuensis gen. nov., sp. nov., a Grampositive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses

Hidetoshi Morita, Chiharu Shiratori, Masaru Murakami, Hideto Takami, Hidehiro Toh, Yukio Kato, Fumihiko Nakajima, Misako Takagi, Hiroaki Akita, Toshio Masaoka, Masahira Hattori

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Four bacterial strains, designated ST18T, HM244, HM250 and DI49, were isolated from the fresh faeces of four thoroughbred horses in Japan. Cells were Gram-positive, strictly anaerobic, catalase-negative, non-spore-forming, non-motile rods that occurred in chains. They were placed in the same subcluster based on 16S rRNA gene sequence analysis, phenotypic characteristics and levels of DNA-DNA relatedness. Their DNA G+C content ranged from 36 to 38 mol%. Lactobacillus catenaformis, Lactobacillus vitulinus and Catenibacterium mitsuokai belong to cluster XVII of the Clostridium subphylum. Strain ST18T was most closely related to L. catenaformis ATCC 25536T in the phylogenetic tree, but these strains shared only 89.9% (1336/1486 bp) 16S rRNA gene sequence similarity. L. catenaformis, L. vitulinus and C. mitsuokai are homofermentative bacteria, whereas ST18T produced CO2 from glucose. Whereas the cell-wall peptidoglycan type of L. catenaformis and L. vitulinus was L-Lys-L-Ala3, that of C. mitsuokai and the subgroup represented by ST18T was A1γ (L-Ala-D-Glu-meso-diaminopimelic acid). On the basis of 16S rRNA gene sequence divergence of more than 10% from L. catenaformis as well as phenotypic characteristics, strains ST18T, HM244, HM250 and DI49 are considered to represent a novel species of a new genus belonging to the Clostridium subphylum cluster XVII, for which the name Sharpea azabuensis gen. nov., sp. nov. is proposed. The type strain of Sharpea azabuensis is ST18T (=JCM 14210T =DSM 18934T).

Original languageEnglish
Pages (from-to)2682-2686
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number12
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Eggerthia catenaformis
Catenibacterium mitsuokai
Anaerobic Bacteria
Kandleria vitulina
horse
rRNA Genes
Feces
feces
Horses
Clostridium
Lactobacillus
horses
DNA
bacterium
gene
new species
bacteria
Diaminopimelic Acid
ribosomal RNA
nucleotide sequences

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Sharpea azabuensis gen. nov., sp. nov., a Grampositive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses. / Morita, Hidetoshi; Shiratori, Chiharu; Murakami, Masaru; Takami, Hideto; Toh, Hidehiro; Kato, Yukio; Nakajima, Fumihiko; Takagi, Misako; Akita, Hiroaki; Masaoka, Toshio; Hattori, Masahira.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 58, No. 12, 2008, p. 2682-2686.

Research output: Contribution to journalArticle

Morita, Hidetoshi ; Shiratori, Chiharu ; Murakami, Masaru ; Takami, Hideto ; Toh, Hidehiro ; Kato, Yukio ; Nakajima, Fumihiko ; Takagi, Misako ; Akita, Hiroaki ; Masaoka, Toshio ; Hattori, Masahira. / Sharpea azabuensis gen. nov., sp. nov., a Grampositive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses. In: International Journal of Systematic and Evolutionary Microbiology. 2008 ; Vol. 58, No. 12. pp. 2682-2686.
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AU - Takami, Hideto

AU - Toh, Hidehiro

AU - Kato, Yukio

AU - Nakajima, Fumihiko

AU - Takagi, Misako

AU - Akita, Hiroaki

AU - Masaoka, Toshio

AU - Hattori, Masahira

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