Shear-stress effect on mitochondrial membrane potential and albumin uptake in cultured endothelial cells

Susumu Kudo, Ryoma Morigaki, Junichi Saito, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow in vivo and shear stress affects ATP-dependent macromolecular transport in ECs. However, the relationship between the ATP production and shear stress is still unclear. We, therefore, evaluated mitochondrial ATP synthesis activity in cultured endothelial cells exposed to shear stress, using a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolycarbocyanine iodide, JC-1). Low shear stress (10 dyn/cm2) increased mitochondrial membrane potential by 30%. On the contrary, high shear stress (60 dyn/cm2) decreased it by 20%. This observation was consistent with the ATP-dependent albumin uptake into endothelial cells. Our results indicate that ATP synthetic activity is related to the albumin uptake into endothelial cells. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)616-621
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume270
Issue number2
DOIs
Publication statusPublished - 2000 Apr 13
Externally publishedYes

Fingerprint

Mitochondrial Membrane Potential
Endothelial cells
Shear stress
Albumins
Cultured Cells
Endothelial Cells
Adenosine Triphosphate
Membranes
Iodides
Blood vessels
Blood Vessels
Lasers
Microscopes
Blood
Cell Line
Scanning

Keywords

  • ATP
  • Endothelial cell
  • Macromolecule uptake
  • Mitochondrial membrane potential
  • Shear stress

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Shear-stress effect on mitochondrial membrane potential and albumin uptake in cultured endothelial cells. / Kudo, Susumu; Morigaki, Ryoma; Saito, Junichi; Ikeda, Mariko; Oka, Kotaro; Tanishita, Kazuo.

In: Biochemical and Biophysical Research Communications, Vol. 270, No. 2, 13.04.2000, p. 616-621.

Research output: Contribution to journalArticle

Kudo, Susumu ; Morigaki, Ryoma ; Saito, Junichi ; Ikeda, Mariko ; Oka, Kotaro ; Tanishita, Kazuo. / Shear-stress effect on mitochondrial membrane potential and albumin uptake in cultured endothelial cells. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 270, No. 2. pp. 616-621.
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