Si-doping increases the adjuvant activity of hydroxyapatite nanorods

Xiupeng Wang, Shu Ihara, Xia Li, Atsuo Ito, Yu Sogo, Yohei Watanabe, Noriko M. Tsuji, Atsushi Yamazaki

Research output: Contribution to journalArticle

Abstract

Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.

LanguageEnglish
Pages300-307
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume174
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Nanotubes
Durapatite
Nanorods
Hydroxyapatite
nanorods
Doping (additives)
secretions
Immunologic Adjuvants
vaccines
bone marrow
Cytokines
biocompatibility
Recombinant proteins
Vaccines
Biocompatibility
Recombinant Proteins
Silicon Dioxide
Dendritic Cells
silicon dioxide
proteins

Keywords

  • Adjuvant
  • Cytokine secretion
  • Hydroxyapatite
  • Si-doping
  • Th1
  • Th2

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Si-doping increases the adjuvant activity of hydroxyapatite nanorods. / Wang, Xiupeng; Ihara, Shu; Li, Xia; Ito, Atsuo; Sogo, Yu; Watanabe, Yohei; Tsuji, Noriko M.; Yamazaki, Atsushi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 174, 01.02.2019, p. 300-307.

Research output: Contribution to journalArticle

Wang, Xiupeng ; Ihara, Shu ; Li, Xia ; Ito, Atsuo ; Sogo, Yu ; Watanabe, Yohei ; Tsuji, Noriko M. ; Yamazaki, Atsushi. / Si-doping increases the adjuvant activity of hydroxyapatite nanorods. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 174. pp. 300-307.
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