Abstract
Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.
| Language | English |
|---|---|
| Pages | 300-307 |
| Number of pages | 8 |
| Journal | Colloids and Surfaces B: Biointerfaces |
| Volume | 174 |
| DOIs | |
| Publication status | Published - 2019 Feb 1 |
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Keywords
- Adjuvant
- Cytokine secretion
- Hydroxyapatite
- Si-doping
- Th1
- Th2
ASJC Scopus subject areas
- Biotechnology
- Surfaces and Interfaces
- Physical and Theoretical Chemistry
- Colloid and Surface Chemistry
Cite this
Si-doping increases the adjuvant activity of hydroxyapatite nanorods. / Wang, Xiupeng; Ihara, Shu; Li, Xia; Ito, Atsuo; Sogo, Yu; Watanabe, Yohei; Tsuji, Noriko M.; Yamazaki, Atsushi.
In: Colloids and Surfaces B: Biointerfaces, Vol. 174, 01.02.2019, p. 300-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Si-doping increases the adjuvant activity of hydroxyapatite nanorods
AU - Wang, Xiupeng
AU - Ihara, Shu
AU - Li, Xia
AU - Ito, Atsuo
AU - Sogo, Yu
AU - Watanabe, Yohei
AU - Tsuji, Noriko M.
AU - Yamazaki, Atsushi
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.
AB - Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.
KW - Adjuvant
KW - Cytokine secretion
KW - Hydroxyapatite
KW - Si-doping
KW - Th1
KW - Th2
UR - http://www.scopus.com/inward/record.url?scp=85056748005&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056748005&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.11.026
DO - 10.1016/j.colsurfb.2018.11.026
M3 - Article
VL - 174
SP - 300
EP - 307
JO - Colloids and Surfaces B: Biointerfaces
T2 - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -