Si-doping increases the adjuvant activity of hydroxyapatite nanorods

Xiupeng Wang, Shu Ihara, Xia Li, Atsuo Ito, Yu Sogo, Yohei Watanabe, Noriko M. Tsuji, Atsushi Yamazaki

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.

    Original languageEnglish
    Pages (from-to)300-307
    Number of pages8
    JournalColloids and Surfaces B: Biointerfaces
    Volume174
    DOIs
    Publication statusPublished - 2019 Feb 1

    Fingerprint

    Nanotubes
    Durapatite
    Nanorods
    Hydroxyapatite
    nanorods
    Doping (additives)
    secretions
    Immunologic Adjuvants
    vaccines
    bone marrow
    Cytokines
    biocompatibility
    Recombinant proteins
    Vaccines
    Biocompatibility
    Recombinant Proteins
    Silicon Dioxide
    Dendritic Cells
    silicon dioxide
    proteins

    Keywords

    • Adjuvant
    • Cytokine secretion
    • Hydroxyapatite
    • Si-doping
    • Th1
    • Th2

    ASJC Scopus subject areas

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

    Cite this

    Si-doping increases the adjuvant activity of hydroxyapatite nanorods. / Wang, Xiupeng; Ihara, Shu; Li, Xia; Ito, Atsuo; Sogo, Yu; Watanabe, Yohei; Tsuji, Noriko M.; Yamazaki, Atsushi.

    In: Colloids and Surfaces B: Biointerfaces, Vol. 174, 01.02.2019, p. 300-307.

    Research output: Contribution to journalArticle

    Wang, Xiupeng ; Ihara, Shu ; Li, Xia ; Ito, Atsuo ; Sogo, Yu ; Watanabe, Yohei ; Tsuji, Noriko M. ; Yamazaki, Atsushi. / Si-doping increases the adjuvant activity of hydroxyapatite nanorods. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 174. pp. 300-307.
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    abstract = "Recombinant protein-based vaccines generally show limited immunogenicity and need adjuvants to achieve robust immune responses. Herein, to combine the excellent biocompatibility of hydroxyapatite (HA) and exciting adjuvant activity of silica, Si-doped HA nanorods with Si/P molar ratio from 0 to 0.65 were hydrothermally synthesized and evaluated as immunoadjuvants. Si-doping decreases the size and increases the BET surface area of the nanorods. Si-doping in HA nanorods increases the in vitro adjuvant activity, including CD11c+CD86+ expression and cytokine secretion of bone marrow derived dendritic cells (BMDCs). Moreover, Si-doping in HA increases the ex vivo adjuvant activity as shown by the increase in both Th1 and Th2 cytokines secretion. Si-doped HA nanorods are promising as a new immunoadjuvant.",
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    AU - Li, Xia

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    AU - Sogo, Yu

    AU - Watanabe, Yohei

    AU - Tsuji, Noriko M.

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