Siderophore production of a periplasmic transport binding protein kinase gene defective mutant of Magnetospirillum magneticum AMB-1

Ronie J. Calugay, Yoshiko Okamura, Aris Tri Wahyudi, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A non-magnetic mutant, NMA61, of the magnetic bacterium Magnetospirillum magneticum AMB-1 was generated by transposon mutagenesis to identify genes involved in magnetosome synthesis. The genomic region of NMA61 interrupted by a Mini-Tn5 transposon was analyzed. The transposon was inserted in an open reading frame (ORF) coding for a periplasmic transport binding protein kinase gene homologue. Three adjacent ORFs and a promoter were identified upstream, indicating that the sequences comprised an operon. Phenotype characterizations showed that the growth inhibition imposed by the exogenous non-assimilable iron chelator nitrilotriacetate was relieved in wild type but not in NMA61, by the addition of the isolated wild type siderophore. Higher concentration of siderophores accumulated in the culture medium of NMA61 than in wild type. These data suggest that the interrupted periplasmic transport binding protein kinase gene homologue is required for siderophore transport into M. magneticum AMB-1.

Original languageEnglish
Pages (from-to)852-857
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume323
Issue number3
DOIs
Publication statusPublished - 2004 Oct 22
Externally publishedYes

Fingerprint

Magnetospirillum
Periplasmic Binding Proteins
Siderophores
Protein Kinases
Carrier Proteins
Genes
Open Reading Frames
Magnetosomes
Mutagenesis
Operon
Chelating Agents
Culture Media
Bacteria
Iron
Phenotype
Growth

Keywords

  • Magnetosomes
  • Magnetospirillum magneticum AMB-1
  • Non-magnetic mutant
  • Siderophores

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Siderophore production of a periplasmic transport binding protein kinase gene defective mutant of Magnetospirillum magneticum AMB-1. / Calugay, Ronie J.; Okamura, Yoshiko; Wahyudi, Aris Tri; Takeyama, Haruko; Matsunaga, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 323, No. 3, 22.10.2004, p. 852-857.

Research output: Contribution to journalArticle

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