Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant

Sho Hideshima, Keisuke Fujita, Yoshitaka Harada, Mika Tsuna, Yasuhiro Seto, Satoshi Sekiguchi, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Food allergens, especially buckwheat proteins, sometimes induce anaphylactic shock in patients after ingestion. Development of a simple and rapid screening method based on a field effect transistor (FET) biosensor for food allergens in food facilities or products is in demand. In this study, we achieved the FET detection of a buckwheat allergenic protein (BWp16), which is not charged enough to be electrically detected by FET biosensors, by introducing additional negative charges from anionic surfactants to the target proteins. A change in the FET characteristics reflecting surface potential caused by the adsorption of target charged proteins was observed when the target sample was coupled with the anionic surfactant (sodium dodecyl sulfate; SDS), while no significant response was detected without any surfactant treatment. It was suggested that the surfactant conjugated with the protein could be useful for the charge amplification of the target proteins. The surface plasmon resonance analysis revealed that the SDS-coupled proteins were successfully captured by the receptors immobilized on the sensing surface. Additionally, we obtained the FET responses at various concentrations of BWp16 ranging from 1 ng/mL to 10 μg/mL. These results suggest that a signal amplification method for FET biosensing is useful for allergen detection in the food industry.

Original languageEnglish
Pages (from-to)90-94
Number of pages5
JournalSensing and Bio-Sensing Research
Volume7
DOIs
Publication statusPublished - 2016 Mar 1

Fingerprint

Fagopyrum
Anionic surfactants
Biosensing Techniques
Field effect transistors
Biosensors
Surface-Active Agents
Amplification
Proteins
Allergens
Food
Surface active agents
Surface Plasmon Resonance
Food Industry
Sodium dodecyl sulfate
Surface potential
Surface plasmon resonance
Anaphylaxis
Sodium Dodecyl Sulfate
Adsorption
Screening

Keywords

  • Field effect transistor biosensor
  • Food allergen
  • Intrinsic charge
  • Ionic surfactant
  • Signal amplification

ASJC Scopus subject areas

  • Biotechnology
  • Signal Processing
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

Cite this

Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant. / Hideshima, Sho; Fujita, Keisuke; Harada, Yoshitaka; Tsuna, Mika; Seto, Yasuhiro; Sekiguchi, Satoshi; Kuroiwa, Shigeki; Nakanishi, Takuya; Osaka, Tetsuya.

In: Sensing and Bio-Sensing Research, Vol. 7, 01.03.2016, p. 90-94.

Research output: Contribution to journalArticle

Hideshima, Sho ; Fujita, Keisuke ; Harada, Yoshitaka ; Tsuna, Mika ; Seto, Yasuhiro ; Sekiguchi, Satoshi ; Kuroiwa, Shigeki ; Nakanishi, Takuya ; Osaka, Tetsuya. / Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant. In: Sensing and Bio-Sensing Research. 2016 ; Vol. 7. pp. 90-94.
@article{790190027ade4799a0355818d7301bc0,
title = "Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant",
abstract = "Food allergens, especially buckwheat proteins, sometimes induce anaphylactic shock in patients after ingestion. Development of a simple and rapid screening method based on a field effect transistor (FET) biosensor for food allergens in food facilities or products is in demand. In this study, we achieved the FET detection of a buckwheat allergenic protein (BWp16), which is not charged enough to be electrically detected by FET biosensors, by introducing additional negative charges from anionic surfactants to the target proteins. A change in the FET characteristics reflecting surface potential caused by the adsorption of target charged proteins was observed when the target sample was coupled with the anionic surfactant (sodium dodecyl sulfate; SDS), while no significant response was detected without any surfactant treatment. It was suggested that the surfactant conjugated with the protein could be useful for the charge amplification of the target proteins. The surface plasmon resonance analysis revealed that the SDS-coupled proteins were successfully captured by the receptors immobilized on the sensing surface. Additionally, we obtained the FET responses at various concentrations of BWp16 ranging from 1 ng/mL to 10 μg/mL. These results suggest that a signal amplification method for FET biosensing is useful for allergen detection in the food industry.",
keywords = "Field effect transistor biosensor, Food allergen, Intrinsic charge, Ionic surfactant, Signal amplification",
author = "Sho Hideshima and Keisuke Fujita and Yoshitaka Harada and Mika Tsuna and Yasuhiro Seto and Satoshi Sekiguchi and Shigeki Kuroiwa and Takuya Nakanishi and Tetsuya Osaka",
year = "2016",
month = "3",
day = "1",
doi = "10.1016/j.sbsr.2016.01.011",
language = "English",
volume = "7",
pages = "90--94",
journal = "Sensing and Bio-Sensing Research",
issn = "2214-1804",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Signal amplification in electrochemical detection of buckwheat allergenic protein using field effect transistor biosensor by introduction of anionic surfactant

AU - Hideshima, Sho

AU - Fujita, Keisuke

AU - Harada, Yoshitaka

AU - Tsuna, Mika

AU - Seto, Yasuhiro

AU - Sekiguchi, Satoshi

AU - Kuroiwa, Shigeki

AU - Nakanishi, Takuya

AU - Osaka, Tetsuya

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Food allergens, especially buckwheat proteins, sometimes induce anaphylactic shock in patients after ingestion. Development of a simple and rapid screening method based on a field effect transistor (FET) biosensor for food allergens in food facilities or products is in demand. In this study, we achieved the FET detection of a buckwheat allergenic protein (BWp16), which is not charged enough to be electrically detected by FET biosensors, by introducing additional negative charges from anionic surfactants to the target proteins. A change in the FET characteristics reflecting surface potential caused by the adsorption of target charged proteins was observed when the target sample was coupled with the anionic surfactant (sodium dodecyl sulfate; SDS), while no significant response was detected without any surfactant treatment. It was suggested that the surfactant conjugated with the protein could be useful for the charge amplification of the target proteins. The surface plasmon resonance analysis revealed that the SDS-coupled proteins were successfully captured by the receptors immobilized on the sensing surface. Additionally, we obtained the FET responses at various concentrations of BWp16 ranging from 1 ng/mL to 10 μg/mL. These results suggest that a signal amplification method for FET biosensing is useful for allergen detection in the food industry.

AB - Food allergens, especially buckwheat proteins, sometimes induce anaphylactic shock in patients after ingestion. Development of a simple and rapid screening method based on a field effect transistor (FET) biosensor for food allergens in food facilities or products is in demand. In this study, we achieved the FET detection of a buckwheat allergenic protein (BWp16), which is not charged enough to be electrically detected by FET biosensors, by introducing additional negative charges from anionic surfactants to the target proteins. A change in the FET characteristics reflecting surface potential caused by the adsorption of target charged proteins was observed when the target sample was coupled with the anionic surfactant (sodium dodecyl sulfate; SDS), while no significant response was detected without any surfactant treatment. It was suggested that the surfactant conjugated with the protein could be useful for the charge amplification of the target proteins. The surface plasmon resonance analysis revealed that the SDS-coupled proteins were successfully captured by the receptors immobilized on the sensing surface. Additionally, we obtained the FET responses at various concentrations of BWp16 ranging from 1 ng/mL to 10 μg/mL. These results suggest that a signal amplification method for FET biosensing is useful for allergen detection in the food industry.

KW - Field effect transistor biosensor

KW - Food allergen

KW - Intrinsic charge

KW - Ionic surfactant

KW - Signal amplification

UR - http://www.scopus.com/inward/record.url?scp=84961778296&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961778296&partnerID=8YFLogxK

U2 - 10.1016/j.sbsr.2016.01.011

DO - 10.1016/j.sbsr.2016.01.011

M3 - Article

AN - SCOPUS:84961778296

VL - 7

SP - 90

EP - 94

JO - Sensing and Bio-Sensing Research

JF - Sensing and Bio-Sensing Research

SN - 2214-1804

ER -