Significance of cell electrokinetic properties determined by soft-particle analysis in bacterial adhesion onto a solid surface

Satoshi Tsuneda, Hirotoshi Aikawa, Hiroshi Hayashi, Akira Hirata

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    The influence of extracellular polymeric substances (EPSs) on bacterial cell electrokinetic properties and on cell adhesion onto glass beads in connection with bacterial cell electrokinetic properties was investigated using 12 heterotrophic bacterial strains. Bacterial cell surface properties such as the softness 1/λ and charge density ZN were determined by Ohshima's soft-particle analysis using the measured electrophoretic mobility as a function of ionic strength. In 10 of 12 strains, when EPSs covering the cell surface were removed, the softness of the cell decreased, indicating that EPS adsorption enhanced the ease of liquid fluid in the ion-penetrable layer on the cell surface. On the other hand, the negative charge density of the cell surface increased for 9 of 12 strains, suggesting that EPSs covering the cell surface decreased the negative charge density of the cell surface layer. In addition, the characteristics of bacterial cell adhesion onto glass beads were evaluated by the packed-bed method and the data were interpreted to indicate cell adhesiveness. As a result, the efficiency of cell adhesion onto glass beads increased as negative cell surface potential ψ0 decreased, whereas there seemed to be no correlation between zeta potential and cell adhesiveness. Cell surface potential ψ0, which was derived by taking the bacterial polymer layer with EPSs into consideration, provided a more detailed understanding of the electrokinetic properties of bacterial cells.

    Original languageEnglish
    Pages (from-to)410-417
    Number of pages8
    JournalJournal of Colloid and Interface Science
    Volume279
    Issue number2
    DOIs
    Publication statusPublished - 2004 Nov 15

    Fingerprint

    electrokinetics
    solid surfaces
    adhesion
    Adhesion
    Cell adhesion
    Charge density
    cells
    Surface potential
    Glass
    Electrophoretic mobility
    Packed beds
    Zeta potential
    Ionic strength
    Surface properties
    beads
    Polymers
    softness
    Ions
    Adsorption
    Fluids

    Keywords

    • Bacterial adhesion
    • Electrophoretic mobility
    • Extracellular polymeric substance (EPS)
    • Soft-particle electrophoresis theory
    • Zeta potential

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry
    • Surfaces and Interfaces

    Cite this

    Significance of cell electrokinetic properties determined by soft-particle analysis in bacterial adhesion onto a solid surface. / Tsuneda, Satoshi; Aikawa, Hirotoshi; Hayashi, Hiroshi; Hirata, Akira.

    In: Journal of Colloid and Interface Science, Vol. 279, No. 2, 15.11.2004, p. 410-417.

    Research output: Contribution to journalArticle

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    abstract = "The influence of extracellular polymeric substances (EPSs) on bacterial cell electrokinetic properties and on cell adhesion onto glass beads in connection with bacterial cell electrokinetic properties was investigated using 12 heterotrophic bacterial strains. Bacterial cell surface properties such as the softness 1/λ and charge density ZN were determined by Ohshima's soft-particle analysis using the measured electrophoretic mobility as a function of ionic strength. In 10 of 12 strains, when EPSs covering the cell surface were removed, the softness of the cell decreased, indicating that EPS adsorption enhanced the ease of liquid fluid in the ion-penetrable layer on the cell surface. On the other hand, the negative charge density of the cell surface increased for 9 of 12 strains, suggesting that EPSs covering the cell surface decreased the negative charge density of the cell surface layer. In addition, the characteristics of bacterial cell adhesion onto glass beads were evaluated by the packed-bed method and the data were interpreted to indicate cell adhesiveness. As a result, the efficiency of cell adhesion onto glass beads increased as negative cell surface potential ψ0 decreased, whereas there seemed to be no correlation between zeta potential and cell adhesiveness. Cell surface potential ψ0, which was derived by taking the bacterial polymer layer with EPSs into consideration, provided a more detailed understanding of the electrokinetic properties of bacterial cells.",
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