Significance of structural variation in thylakoid membranes in maintaining functional photosystems during reproductive growth

Hatsumi Nozue, Kaori Oono, Yoshinobu Ichikawa, Shun Tanimura, Kana Shirai, Kintake Sonoike, Masayuki Nozue, Nobuaki Hayashida

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Structural variation in the stroma-grana (SG) arrangement of the thylakoid membranes, such as changes in the thickness of the grana stacks and in the ratio between grana and inter-grana thylakoid, is often observed. Broadly, such alterations are considered acclimation to changes in growth and the environment. However, the relation of thylakoid morphology to plant growth and photosynthesis remains obscure. Here, we report changes in the thylakoid during leaf development under a fixed light condition. Histological studies on the chloroplasts of fresh green Arabidopsis leaves have shown that characteristically shaped thylakoid membranes lacking the inter-grana region, referred to hereafter as isolated-grana (IG), occurred adjacent to highly ordered, large grana layers. This morphology was restored to conventional SG thylakoid membranes with the removal of bolting stems from reproductive plants. Statistical analysis showed a negative correlation between the incidences of IG-type chloroplasts in mesophyll cells and the rates of leaf growth. Fluorescence parameters calculated from pulse-amplitude modulated fluorometry measurements and CO2 assimilation data showed that the IG thylakoids had a photosynthetic ability that was equivalent to that of the SG thylakoids under moderate light. However, clear differences were observed in the chlorophyll a/b ratio. The IG thylakoids were apparently an acclimated phenotype to the internal condition of source leaves. The idea is supported by the fact that the life span of the IG thylakoids increased significantly in the later developing leaves. In conclusion, the heterogeneous state of thylakoid membranes is likely important in maintaining photosynthesis during the reproductive phase of growth.

    Original languageEnglish
    Pages (from-to)111-123
    Number of pages13
    JournalPhysiologia Plantarum
    Volume160
    Issue number1
    DOIs
    Publication statusPublished - 2017 May 1

    Fingerprint

    grana
    Thylakoids
    thylakoids
    Growth
    leaves
    chloroplasts
    photosynthesis
    chlorophyll
    bolting
    fluorometry
    Photosynthesis
    Chloroplasts
    green leafy vegetables
    leaf development
    mesophyll
    assimilation (physiology)
    acclimation

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science
    • Cell Biology

    Cite this

    Significance of structural variation in thylakoid membranes in maintaining functional photosystems during reproductive growth. / Nozue, Hatsumi; Oono, Kaori; Ichikawa, Yoshinobu; Tanimura, Shun; Shirai, Kana; Sonoike, Kintake; Nozue, Masayuki; Hayashida, Nobuaki.

    In: Physiologia Plantarum, Vol. 160, No. 1, 01.05.2017, p. 111-123.

    Research output: Contribution to journalArticle

    Nozue, Hatsumi ; Oono, Kaori ; Ichikawa, Yoshinobu ; Tanimura, Shun ; Shirai, Kana ; Sonoike, Kintake ; Nozue, Masayuki ; Hayashida, Nobuaki. / Significance of structural variation in thylakoid membranes in maintaining functional photosystems during reproductive growth. In: Physiologia Plantarum. 2017 ; Vol. 160, No. 1. pp. 111-123.
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