Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor

Tatsuya Saeki, Yuriko Sugamura, Masahito Hosokawa, Tomoko Yoshino, Tae kyu Lim, Manabu Harada, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64mm2) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.

Original languageEnglish
Pages (from-to)350-355
Number of pages6
JournalBiosensors and Bioelectronics
Volume67
DOIs
Publication statusPublished - 2015 May 5
Externally publishedYes

Fingerprint

CD4-CD8 Ratio
Microcavities
Imaging systems
Leukocytes
Blood
Testing
Signal-To-Noise Ratio
Fingers
Blood Cells
Acquired Immunodeficiency Syndrome
Color
HIV
T-Lymphocytes
Imaging techniques
Light
T-cells
Signal to noise ratio
Cells
Irradiation
Substrates

Keywords

  • 2D photosensor
  • CD4 testing
  • HIV/AIDS treatment
  • Large-field imaging system
  • Microcavity array

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor. / Saeki, Tatsuya; Sugamura, Yuriko; Hosokawa, Masahito; Yoshino, Tomoko; Lim, Tae kyu; Harada, Manabu; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Biosensors and Bioelectronics, Vol. 67, 05.05.2015, p. 350-355.

Research output: Contribution to journalArticle

Saeki, Tatsuya ; Sugamura, Yuriko ; Hosokawa, Masahito ; Yoshino, Tomoko ; Lim, Tae kyu ; Harada, Manabu ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor. In: Biosensors and Bioelectronics. 2015 ; Vol. 67. pp. 350-355.
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