Abstract
This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64mm2) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 350-355 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 67 |
| DOIs | |
| Publication status | Published - 2015 May 5 |
| Externally published | Yes |
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Keywords
- 2D photosensor
- CD4 testing
- HIV/AIDS treatment
- Large-field imaging system
- Microcavity array
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry
Cite this
Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor. / Saeki, Tatsuya; Sugamura, Yuriko; Hosokawa, Masahito; Yoshino, Tomoko; Lim, Tae kyu; Harada, Manabu; Matsunaga, Tadashi; Tanaka, Tsuyoshi.
In: Biosensors and Bioelectronics, Vol. 67, 05.05.2015, p. 350-355.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor
AU - Saeki, Tatsuya
AU - Sugamura, Yuriko
AU - Hosokawa, Masahito
AU - Yoshino, Tomoko
AU - Lim, Tae kyu
AU - Harada, Manabu
AU - Matsunaga, Tadashi
AU - Tanaka, Tsuyoshi
PY - 2015/5/5
Y1 - 2015/5/5
N2 - This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64mm2) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
AB - This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64mm2) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.
KW - 2D photosensor
KW - CD4 testing
KW - HIV/AIDS treatment
KW - Large-field imaging system
KW - Microcavity array
UR - http://www.scopus.com/inward/record.url?scp=84922264901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922264901&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.08.051
DO - 10.1016/j.bios.2014.08.051
M3 - Article
VL - 67
SP - 350
EP - 355
JO - Biosensors
JF - Biosensors
SN - 0956-5663
ER -