Simple estimation of the differential absorption coefficient of P-700 in detergent-treated preparations

Kintake Sonoike, Sakae Katoh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The apparent differential absorption coefficient of P-700 in the thylakoid membranes and Photosystem I particles from the cyanobacterium Synechococcus elongatus was differently affected by various detergents. Triton X-100 and sodium dodecyl sulfate increased the differential absorption coefficient, whereas the absorption value was reduced by digitonin and octyl-d-glucoside. Irrespective of detergents used, however, variations of the differential absorption coefficient were well related to band shifts of chlorophyll a molecules at 690 nm. The band shift also affected, in opposite directions, the two bleaching bands at 680 nm and 700 nm of the P-700 difference spectrum. Thus the differential absorption coefficient of P-700 in detergent-treated preparations can be estimated by measuring the ratio of the 680 nm to 700 nm bands.

Original languageEnglish
Pages (from-to)210-213
Number of pages4
JournalBBA - Bioenergetics
Volume976
Issue number2-3
DOIs
Publication statusPublished - 1989 Sep 28
Externally publishedYes

Fingerprint

Detergents
Photosystem I Protein Complex
Digitonin
Octoxynol
Glucosides
Bleaching
Sodium Dodecyl Sulfate
Synechococcus
Thylakoids
Membranes
Cyanobacteria
Molecules
chlorophyll P 700
Direction compound
chlorophyll a

Keywords

  • (S. elongaius)
  • Detergent
  • Differential absorption coefficient
  • P-700
  • Photosystem I

ASJC Scopus subject areas

  • Biophysics

Cite this

Simple estimation of the differential absorption coefficient of P-700 in detergent-treated preparations. / Sonoike, Kintake; Katoh, Sakae.

In: BBA - Bioenergetics, Vol. 976, No. 2-3, 28.09.1989, p. 210-213.

Research output: Contribution to journalArticle

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