Simple method for quantitative PCR using flow-through PCR device

T. Fukuba, M. Hiraga, Atsuko Takamatsu, C. Provin, T. Yamamoto, T. Fujii

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

    Original languageEnglish
    Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    PublisherChemical and Biological Microsystems Society
    Pages1743-1745
    Number of pages3
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    Duration: 2008 Oct 122008 Oct 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    CitySan Diego, CA
    Period08/10/1208/10/16

    Fingerprint

    Amplification
    Microfluidics
    Fluorescence
    Calibration

    Keywords

    • Flow-through polymerase chain reaction
    • Polydimethylsiloxane
    • Quantitative polymerase chain reaction

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Fukuba, T., Hiraga, M., Takamatsu, A., Provin, C., Yamamoto, T., & Fujii, T. (2008). Simple method for quantitative PCR using flow-through PCR device. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1743-1745). Chemical and Biological Microsystems Society.

    Simple method for quantitative PCR using flow-through PCR device. / Fukuba, T.; Hiraga, M.; Takamatsu, Atsuko; Provin, C.; Yamamoto, T.; Fujii, T.

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1743-1745.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Fukuba, T, Hiraga, M, Takamatsu, A, Provin, C, Yamamoto, T & Fujii, T 2008, Simple method for quantitative PCR using flow-through PCR device. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1743-1745, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
    Fukuba T, Hiraga M, Takamatsu A, Provin C, Yamamoto T, Fujii T. Simple method for quantitative PCR using flow-through PCR device. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 1743-1745
    Fukuba, T. ; Hiraga, M. ; Takamatsu, Atsuko ; Provin, C. ; Yamamoto, T. ; Fujii, T. / Simple method for quantitative PCR using flow-through PCR device. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 1743-1745
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    AU - Fujii, T.

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