Simple method for quantitative PCR using flow-through PCR device

T. Fukuba, M. Hiraga, A. Takamatsu, C. Provin, T. Yamamoto, T. Fujii

Research output: Contribution to conferencePaper

Abstract

In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

Original languageEnglish
Pages1743-1745
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Flow-through polymerase chain reaction
  • Polydimethylsiloxane
  • Quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Fukuba, T., Hiraga, M., Takamatsu, A., Provin, C., Yamamoto, T., & Fujii, T. (2008). Simple method for quantitative PCR using flow-through PCR device. 1743-1745. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.