Simultaneous comparison of thrombogenic reactions to different combinations of anticoagulants, activated clotting times, and materials

Mirei Nagai, Kiyotaka Iwasaki, Mitsuo Umezu, Makoto Ozaki

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Thrombogenic reactions under multiple interactions of pharmacological agents, doses, and materials have not been well understood yet. The aim of this study was to investigate the ability to simultaneously compare thrombogenic reactions to different combinations of anticoagulants, doses, and blood-contacting materials, in a single human blood using an in vitro test method. Four venous blood samples were drawn from each of six healthy volunteers into syringes that contained two different amounts of heparin and argatroban to set the activated clotting time (ACT) to approximately 200 or 500 s, respectively. The four blood samples from each volunteer were immediately poured into two clinical-grade extracorporeal circulation tubes: a polyvinyl chloride (PVC) tube and a poly(2-methoxyethyl acrylate)-coated (PMEA) PVC tube. These tubes with an inner diameter of 12.7 mm were rotated at 183 rpm in a 37

    Original languageEnglish
    Pages (from-to)1605-1612
    Number of pages8
    JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
    Volume102
    Issue number8
    DOIs
    Publication statusPublished - 2014 Nov 1

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    Anticoagulants
    Blood
    Polyvinyl Chloride
    Polyvinyl chlorides
    Syringes
    Heparin

    Keywords

    • Activated clotting time
    • Anticoagulant
    • In vitro thrombogenicity test
    • Platelet activation
    • Total plasma protein adsorption

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Biomaterials

    Cite this

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    abstract = "Thrombogenic reactions under multiple interactions of pharmacological agents, doses, and materials have not been well understood yet. The aim of this study was to investigate the ability to simultaneously compare thrombogenic reactions to different combinations of anticoagulants, doses, and blood-contacting materials, in a single human blood using an in vitro test method. Four venous blood samples were drawn from each of six healthy volunteers into syringes that contained two different amounts of heparin and argatroban to set the activated clotting time (ACT) to approximately 200 or 500 s, respectively. The four blood samples from each volunteer were immediately poured into two clinical-grade extracorporeal circulation tubes: a polyvinyl chloride (PVC) tube and a poly(2-methoxyethyl acrylate)-coated (PMEA) PVC tube. These tubes with an inner diameter of 12.7 mm were rotated at 183 rpm in a 37",
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    AU - Iwasaki, Kiyotaka

    AU - Umezu, Mitsuo

    AU - Ozaki, Makoto

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