Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140

Seiichi Tsukamoto, Teruyuki Fujii, Kotaro Oyama, Seine A. Shintani, Togo Shimozawa, Fuyu Kobirumaki-Shimozawa, Shin'ichi Ishiwata, Norio Fukuda

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    In cardiac muscle, contraction is triggered by sarcolemmal depolarization, resulting in an intracellular Ca2+ transient, binding of Ca2+ to troponin, and subsequent cross-bridge formation (excitation-contraction [EC] coupling). Here, we develop a novel experimental system for simultaneous nano-imaging of intracellular Ca2+ dynamics and single sarcomere length (SL) in rat neonatal cardiomyocytes. We achieve this by expressing a fluorescence resonance energy transfer (FRET)-based Ca2+ sensor yellow Cameleon-Nano (YC-Nano) fused to a-actinin in order to localize to the Z disks. We find that, among four different YC-Nanos, a-actinin-YC-Nano140 is best suited for high-precision analysis of EC coupling and a-actinin-YC-Nano140 enables quantitative analyses of intracellular calcium transients and sarcomere dynamics at low and high temperatures, during spontaneous beating and with electrical stimulation. We use this tool to show that calcium transients are synchronized along the length of a myofibril. However, the averaging of SL along myofibrils causes a marked underestimate (~50%) of the magnitude of displacement because of the different timing of individual SL changes, regardless of the absence or presence of positive inotropy (via β-adrenergic stimulation or enhanced actomyosin interaction). Finally, we find that β-adrenergic stimulation with 50 nM isoproterenol accelerated Ca2+ dynamics, in association with an approximately twofold increase in sarcomere lengthening velocity. We conclude that our experimental system has a broad range of potential applications for the unveiling molecular mechanisms of EC coupling in cardiomyocytes at the single sarcomere level.

    Original languageEnglish
    Article number341
    JournalJournal of General Physiology
    Volume148
    Issue number4
    DOIs
    Publication statusPublished - 2016

    Fingerprint

    Sarcomeres
    Cardiac Myocytes
    Calcium
    Actinin
    Excitation Contraction Coupling
    Myofibrils
    Adrenergic Agents
    Actomyosin
    Fluorescence Resonance Energy Transfer
    Troponin
    Isoproterenol
    Electric Stimulation
    yellow cameleon
    Myocardium
    Temperature

    ASJC Scopus subject areas

    • Physiology

    Cite this

    Tsukamoto, S., Fujii, T., Oyama, K., Shintani, S. A., Shimozawa, T., Kobirumaki-Shimozawa, F., ... Fukuda, N. (2016). Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140. Journal of General Physiology, 148(4), [341]. https://doi.org/10.1085/jgp.201611604

    Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140. / Tsukamoto, Seiichi; Fujii, Teruyuki; Oyama, Kotaro; Shintani, Seine A.; Shimozawa, Togo; Kobirumaki-Shimozawa, Fuyu; Ishiwata, Shin'ichi; Fukuda, Norio.

    In: Journal of General Physiology, Vol. 148, No. 4, 341, 2016.

    Research output: Contribution to journalArticle

    Tsukamoto, S, Fujii, T, Oyama, K, Shintani, SA, Shimozawa, T, Kobirumaki-Shimozawa, F, Ishiwata, S & Fukuda, N 2016, 'Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140', Journal of General Physiology, vol. 148, no. 4, 341. https://doi.org/10.1085/jgp.201611604
    Tsukamoto, Seiichi ; Fujii, Teruyuki ; Oyama, Kotaro ; Shintani, Seine A. ; Shimozawa, Togo ; Kobirumaki-Shimozawa, Fuyu ; Ishiwata, Shin'ichi ; Fukuda, Norio. / Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140. In: Journal of General Physiology. 2016 ; Vol. 148, No. 4.
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    AU - Shintani, Seine A.

    AU - Shimozawa, Togo

    AU - Kobirumaki-Shimozawa, Fuyu

    AU - Ishiwata, Shin'ichi

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