Simultaneous measurement of contractile force and field potential of dynamically beating human iPS cell-derived cardiac cell sheet-tissue with flexible electronics

Takashi Ohya, Haruki Ohtomo, Tetsutaro Kikuchi, Daisuke Sasaki, Yohei Kawamura, Katsuhisa Matsuura, Tatsuya Shimizu, Kenjiro Fukuda*, Takao Someya, Shinjiro Umezu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Human induced pluripotent stem (iPS) cell-derived cardiomyocytes are used forin vitropharmacological and pathological studies worldwide. In particular, the functional assessment of cardiac tissues created from iPS cell-derived cardiomyocytes is expected to provide precise prediction of drug effects and thus streamline the process of drug development. However, the current format of electrophysiological and contractile assessment of cardiomyocytes on a rigid substrate is not appropriate for cardiac tissues that beat dynamically. Here, we show a novel simultaneous measurement system for contractile force and extracellular field potential of iPS cell-derived cardiac cell sheet-tissues using 500 nm-thick flexible electronic sheets. It was confirmed that the developed system is applicable for pharmacological studies and assessments of excitation-contraction coupling-related parameters, such as the electro-mechanical window. Our results indicate that flexible electronics with cardiac tissue engineering provide an advanced platform for drug development. This system will contribute to gaining new insight in pharmacological study of human cardiac function.

Original languageEnglish
Pages (from-to)3899-3909
Number of pages11
JournalLab on a Chip
Volume21
Issue number20
DOIs
Publication statusPublished - 2021 Oct 21

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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