Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system

Ippei Inoue, Daisuke Shiomi, Ikuro Kawagishi, Kenji Yasuda

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe the change of the localization of expressed sensor-protein and the motility for generations. Localization of the aspartate sensitive sensor protein at two poles in Escherichia coli decreased quickly after the aspartate was added into the cultivation medium. However, it took more than three generations for recovering the localization after the removal of aspartate from the medium. Moreover, the tumbling frequency was strongly related to the localization of the sensor protein in a cell. The results indicate that the change of the spatial localization of sensor protein, which was inherited for more than three generations, may contribute to cells, motility as the inheritable information.

Original languageEnglish
Article number4
JournalJournal of Nanobiotechnology
Volume2
DOIs
Publication statusPublished - 2004 Apr 30

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Pharmaceutical Science

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