Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor

Kazuaki Hibiya, Akihiko Terada, Satoshi Tsuneda, Akira Hirata

    Research output: Contribution to journalArticle

    167 Citations (Scopus)

    Abstract

    Nitrogen and carbon components in domestic modified wastewater were completely removed by simultaneous nitrification and denitrification using a membrane-aerated biofilm reactor where biofilm was fixed on a hollow-fiber membrane. To measure the spatial distribution of pH, ammonium and nitrate ions and to observe microbes inside the biofilm fixed on the membrane, microelectrodes and the fluorescence in situ hybridization (FISH) method were applied. Due to plug flow in the vertical direction (from the bottom to the top of the reactor), ammonium nitrogen was gradually removed and negligible nitrate nitrogen was detected throughout the reactor. FISH revealed that ammoniaoxidizing bacteria were mainly distributed inside the biofilm and other bacteria, which included denitrifying bacteria, were mainly distributed outside the biofilm and over the suspended sludge. In order to characterize bacterial activity in the vertical direction of the reactor, nitrification rates at lower, central and upper points were calculated using microelectrode data. The nitrification rate at the lower point was 7 and 125 times higher than those at the central and upper points, respectively. These results show that the removal of carbon and nitrogen compounds was accomplished efficiently by using various kinds of bacteria distributed vertically and horizontally in a single reactor.

    Original languageEnglish
    Pages (from-to)23-32
    Number of pages10
    JournalJournal of Biotechnology
    Volume100
    Issue number1
    DOIs
    Publication statusPublished - 2003 Jan 9

    Fingerprint

    Nitrification
    Denitrification
    Biofilms
    Membranes
    Bacteria
    Nitrogen
    Microelectrodes
    Fluorescence In Situ Hybridization
    Nitrates
    Carbon
    Fluorescence
    Nitrogen Compounds
    Nitrogen compounds
    Waste Water
    Sewage
    Ammonium Compounds
    Spatial distribution
    Wastewater
    Ions
    Fibers

    Keywords

    • Carbon and nitrogen removal
    • Fluorescence in situ hybridization
    • Membrane-aerated biofilm reactor
    • Microelectrode
    • Nitrification rate
    • Simultaneous nitrification and denitrification

    ASJC Scopus subject areas

    • Biotechnology

    Cite this

    Simultaneous nitrification and denitrification by controlling vertical and horizontal microenvironment in a membrane-aerated biofilm reactor. / Hibiya, Kazuaki; Terada, Akihiko; Tsuneda, Satoshi; Hirata, Akira.

    In: Journal of Biotechnology, Vol. 100, No. 1, 09.01.2003, p. 23-32.

    Research output: Contribution to journalArticle

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