Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor

Satoshi Tsuneda, Takashi Ohno, Koichi Soejima, Akira Hirata

    Research output: Contribution to journalArticle

    170 Citations (Scopus)

    Abstract

    In this study, an anaerobic/aerobic/anoxic process (referred to as an AOA process) using a sequencing batch reactor (SBR) was proposed for simultaneous phosphorus and nitrogen removal from wastewater. The AOA process was stably operated over more than one year when a certain amount of carbon substrate (40 mg-C/L in a reactor) was supplemented to inhibit aerobic phosphate uptake. The average nitrogen and phosphorus removal efficiencies were 83% and 92%, respectively. It was demonstrated that phosphate-accumulating organisms (PAOs) capable of utilizing nitrite as an electron acceptor, the so-called denitrifying phosphate-accumulating organisms (DNPAOs), could exist in the AOA process. Moreover, the ratio of anoxic phosphate uptake rate (PUR) to aerobic PUR (anoxic/aerobic PUR ratio), which indicates the fraction of DNPAOs in total PAOs, was experimentally evaluated. The results indicate that the AOA process has a much larger anoxic/aerobic PUR ratio than the conventional A2O (anaerobic/anoxic/aerobic) and AO (anaerobic/aerobic) processes. In conclusion, the AOA process allows DNPAOs to take an active part in simultaneous nitrogen and phosphorus removal in an SBR when a suitable amount of carbon substrate is supplied at the start of aerobic conditions.

    Original languageEnglish
    Pages (from-to)191-196
    Number of pages6
    JournalBiochemical Engineering Journal
    Volume27
    Issue number3
    DOIs
    Publication statusPublished - 2006 Jan

    Fingerprint

    Batch reactors
    Phosphorus
    Phosphates
    Nitrogen
    Carbon
    Nitrogen removal
    Substrates
    Nitrites
    Waste Water
    Wastewater
    Electrons

    Keywords

    • Anaerobic/aerobic/anoxic (AOA) process
    • Denitrifying phosphate-accumulating organisms (DNPAOs)
    • Enhanced biological phosphorus removal (EBPR)
    • Sequencing batch reactor (SBR)

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Chemical Engineering(all)

    Cite this

    Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor. / Tsuneda, Satoshi; Ohno, Takashi; Soejima, Koichi; Hirata, Akira.

    In: Biochemical Engineering Journal, Vol. 27, No. 3, 01.2006, p. 191-196.

    Research output: Contribution to journalArticle

    @article{ae51f88f3f9749aaa66a1d06c0c931de,
    title = "Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor",
    abstract = "In this study, an anaerobic/aerobic/anoxic process (referred to as an AOA process) using a sequencing batch reactor (SBR) was proposed for simultaneous phosphorus and nitrogen removal from wastewater. The AOA process was stably operated over more than one year when a certain amount of carbon substrate (40 mg-C/L in a reactor) was supplemented to inhibit aerobic phosphate uptake. The average nitrogen and phosphorus removal efficiencies were 83{\%} and 92{\%}, respectively. It was demonstrated that phosphate-accumulating organisms (PAOs) capable of utilizing nitrite as an electron acceptor, the so-called denitrifying phosphate-accumulating organisms (DNPAOs), could exist in the AOA process. Moreover, the ratio of anoxic phosphate uptake rate (PUR) to aerobic PUR (anoxic/aerobic PUR ratio), which indicates the fraction of DNPAOs in total PAOs, was experimentally evaluated. The results indicate that the AOA process has a much larger anoxic/aerobic PUR ratio than the conventional A2O (anaerobic/anoxic/aerobic) and AO (anaerobic/aerobic) processes. In conclusion, the AOA process allows DNPAOs to take an active part in simultaneous nitrogen and phosphorus removal in an SBR when a suitable amount of carbon substrate is supplied at the start of aerobic conditions.",
    keywords = "Anaerobic/aerobic/anoxic (AOA) process, Denitrifying phosphate-accumulating organisms (DNPAOs), Enhanced biological phosphorus removal (EBPR), Sequencing batch reactor (SBR)",
    author = "Satoshi Tsuneda and Takashi Ohno and Koichi Soejima and Akira Hirata",
    year = "2006",
    month = "1",
    doi = "10.1016/j.bej.2005.07.004",
    language = "English",
    volume = "27",
    pages = "191--196",
    journal = "Biochemical Engineering Journal",
    issn = "1369-703X",
    publisher = "Elsevier",
    number = "3",

    }

    TY - JOUR

    T1 - Simultaneous nitrogen and phosphorus removal using denitrifying phosphate-accumulating organisms in a sequencing batch reactor

    AU - Tsuneda, Satoshi

    AU - Ohno, Takashi

    AU - Soejima, Koichi

    AU - Hirata, Akira

    PY - 2006/1

    Y1 - 2006/1

    N2 - In this study, an anaerobic/aerobic/anoxic process (referred to as an AOA process) using a sequencing batch reactor (SBR) was proposed for simultaneous phosphorus and nitrogen removal from wastewater. The AOA process was stably operated over more than one year when a certain amount of carbon substrate (40 mg-C/L in a reactor) was supplemented to inhibit aerobic phosphate uptake. The average nitrogen and phosphorus removal efficiencies were 83% and 92%, respectively. It was demonstrated that phosphate-accumulating organisms (PAOs) capable of utilizing nitrite as an electron acceptor, the so-called denitrifying phosphate-accumulating organisms (DNPAOs), could exist in the AOA process. Moreover, the ratio of anoxic phosphate uptake rate (PUR) to aerobic PUR (anoxic/aerobic PUR ratio), which indicates the fraction of DNPAOs in total PAOs, was experimentally evaluated. The results indicate that the AOA process has a much larger anoxic/aerobic PUR ratio than the conventional A2O (anaerobic/anoxic/aerobic) and AO (anaerobic/aerobic) processes. In conclusion, the AOA process allows DNPAOs to take an active part in simultaneous nitrogen and phosphorus removal in an SBR when a suitable amount of carbon substrate is supplied at the start of aerobic conditions.

    AB - In this study, an anaerobic/aerobic/anoxic process (referred to as an AOA process) using a sequencing batch reactor (SBR) was proposed for simultaneous phosphorus and nitrogen removal from wastewater. The AOA process was stably operated over more than one year when a certain amount of carbon substrate (40 mg-C/L in a reactor) was supplemented to inhibit aerobic phosphate uptake. The average nitrogen and phosphorus removal efficiencies were 83% and 92%, respectively. It was demonstrated that phosphate-accumulating organisms (PAOs) capable of utilizing nitrite as an electron acceptor, the so-called denitrifying phosphate-accumulating organisms (DNPAOs), could exist in the AOA process. Moreover, the ratio of anoxic phosphate uptake rate (PUR) to aerobic PUR (anoxic/aerobic PUR ratio), which indicates the fraction of DNPAOs in total PAOs, was experimentally evaluated. The results indicate that the AOA process has a much larger anoxic/aerobic PUR ratio than the conventional A2O (anaerobic/anoxic/aerobic) and AO (anaerobic/aerobic) processes. In conclusion, the AOA process allows DNPAOs to take an active part in simultaneous nitrogen and phosphorus removal in an SBR when a suitable amount of carbon substrate is supplied at the start of aerobic conditions.

    KW - Anaerobic/aerobic/anoxic (AOA) process

    KW - Denitrifying phosphate-accumulating organisms (DNPAOs)

    KW - Enhanced biological phosphorus removal (EBPR)

    KW - Sequencing batch reactor (SBR)

    UR - http://www.scopus.com/inward/record.url?scp=27444447638&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=27444447638&partnerID=8YFLogxK

    U2 - 10.1016/j.bej.2005.07.004

    DO - 10.1016/j.bej.2005.07.004

    M3 - Article

    AN - SCOPUS:27444447638

    VL - 27

    SP - 191

    EP - 196

    JO - Biochemical Engineering Journal

    JF - Biochemical Engineering Journal

    SN - 1369-703X

    IS - 3

    ER -