Single administration effects of ethanol on the distribution of white blood cells in rats

Yu Kawashima, Yui Someya, Ken Shirato, Shogo Sato, Hisashi Ideno, Kyoko Kobayashi, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Acute single administration effects of ethanol on the distribution of total white blood cells (WBCs), neutrophil, basophil, eosinophil, monocyte and lymphocytes were studied in rats. Acute single administration effects of ethanol on the number of red blood cells (RBCs), hemoglobin concentration and hematocrit were also examined. Male 8-week-old Sprague Dawley rats were randomly divided into the ethanol-administered (ETA) group and the control (CON) group. Two parts of an experiment, 1) 1st experiment: (ethanol dose: 1.0 g/kg body weight), and 2) 2nd experiment: (ethanol dose: 2.0 g/kg body weight) were carried out in rats. The rats were starved to 19:00, and deprived of diet for 12 hr and water for 1 hr before the single administration of ethanol. 1.0 or 2.0 g/kg body weight of ethanol (in 20% (w/w) ethanol) was orally administered to ETA group rats via a stainless stomach tube. In the CON group rats, 0.9% NaCl solution was orally given with the solution volume being equal, in the same manner. Single administration of 1.0 or 2.0 g/kg body weight of ethanol did not change the number of RBCs, hemoglobin concentration and hematocrit. Single administration of 1.0 g/kg body weight of ethanol did not also change the number of WBCs. However, administration of 2.0 g/kg body weight of ethanol increased significantly the number of neutrophil, basophil, monocyte and total WBCs without changing the number of eosinophil and lymphocytes. These results suggest that single administration of 2.0 g/kg body weight of ethanol to rats increased markedly the number of the natural immunity cells without changing the number of acquired cells.

    Original languageEnglish
    Pages (from-to)347-355
    Number of pages9
    JournalJournal of Toxicological Sciences
    Volume36
    Issue number3
    DOIs
    Publication statusPublished - 2011 Jun

    Fingerprint

    Rats
    Leukocytes
    Blood
    Ethanol
    Cells
    Body Weight
    Lymphocytes
    Basophils
    Hematocrit
    Eosinophils
    Monocytes
    Hemoglobins
    Neutrophils
    Erythrocytes
    Control Groups
    Experiments
    Lymphocyte Count
    Nutrition
    Innate Immunity
    Sprague Dawley Rats

    Keywords

    • Basophil
    • Ethanol
    • Lymphocytes
    • Neutrophil
    • White blood cells

    ASJC Scopus subject areas

    • Toxicology

    Cite this

    Kawashima, Y., Someya, Y., Shirato, K., Sato, S., Ideno, H., Kobayashi, K., ... Imaizumi, K. (2011). Single administration effects of ethanol on the distribution of white blood cells in rats. Journal of Toxicological Sciences, 36(3), 347-355. https://doi.org/10.2131/jts.36.347

    Single administration effects of ethanol on the distribution of white blood cells in rats. / Kawashima, Yu; Someya, Yui; Shirato, Ken; Sato, Shogo; Ideno, Hisashi; Kobayashi, Kyoko; Tachiyashiki, Kaoru; Imaizumi, Kazuhiko.

    In: Journal of Toxicological Sciences, Vol. 36, No. 3, 06.2011, p. 347-355.

    Research output: Contribution to journalArticle

    Kawashima, Y, Someya, Y, Shirato, K, Sato, S, Ideno, H, Kobayashi, K, Tachiyashiki, K & Imaizumi, K 2011, 'Single administration effects of ethanol on the distribution of white blood cells in rats', Journal of Toxicological Sciences, vol. 36, no. 3, pp. 347-355. https://doi.org/10.2131/jts.36.347
    Kawashima, Yu ; Someya, Yui ; Shirato, Ken ; Sato, Shogo ; Ideno, Hisashi ; Kobayashi, Kyoko ; Tachiyashiki, Kaoru ; Imaizumi, Kazuhiko. / Single administration effects of ethanol on the distribution of white blood cells in rats. In: Journal of Toxicological Sciences. 2011 ; Vol. 36, No. 3. pp. 347-355.
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