Single bout of running exercise changes LC3-II expression in rat cardiac muscle

Yuji Ogura*, Motoyuki Iemitsu, Hisashi Naito, Ryo Kakigi, Chiaki Kakehashi, Seiji Maeda, Tatsuo Akema

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Macroautophagy (autophagy) is an intracellular catalytic process. We examined the effect of running exercise, which stimulates cardiac work physiologically, on the expression of microtubule-associated protein 1 light chain 3 (LC3)-II, an indicator of autophagy, as well as some autophagy-related proteins in rat cardiac muscle. The left ventricles were taken from rats immediately (0. h), and at 0.5. h, 1. h or 3. h after a single bout of running exercise on a treadmill for 30. min and also from rats in a rest condition. In these samples, we evaluated the level of LC3-II and p62, and the phosphorylation level of mammalian target of rapamycin (mTOR), Akt and AMP-activated protein kinase alpha (AMPKα) by Western blotting. The exercise produced a biphasic change in LC3-II, with an initial decrease observed immediately after the exercise and a subsequent increase 1. h thereafter. LC3-II then returned to the rest level at 3. h after the exercise. A negative correlation was found between the LC3-II expression and mTOR phosphorylation, which plays a role in inhibiting autophagy. The exercise increased phosphorylation of AMPKα, which stimulates autophagy via suppression of mTOR phosphorylation, immediately after exercise. The level of p62 and phosphorylated Akt was not altered significantly by the exercise. These results suggest for the first time that a single bout of running exercise induces a biphasic change in autophagy in the cardiac muscle. The exercise-induced change in autophagy might be partially mediated by mTOR in the cardiac muscle.

Original languageEnglish
Pages (from-to)756-760
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume414
Issue number4
DOIs
Publication statusPublished - 2011 Nov 4
Externally publishedYes

Keywords

  • Cardiac work
  • Endurance exercise
  • Protein turnover
  • Proteolysis
  • Western blotting

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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