Single cell analysis of the proinflammatory responses of mast cell by a real time secretion assay

Y. Shirasaki, A. Nakahara, N. Shimura, N. Suzuki, M. Yamagishi, J. Mizuno, S. Shoji, O. Ohara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory responses were monitored as cytokine secretion along time. mRNA levels of cytokines by single-cell quantitative PCR was also measured after the secretion assay. The results showed that each of stimulated mast cells is quantitatively and qualitatively distinguishable from each other in terms of cytokine secretion and their mRNA profile.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages843-845
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

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Keywords

  • CYTOP
  • Proinflammatory response
  • Secretion monitoring assay
  • Single-cell

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Shirasaki, Y., Nakahara, A., Shimura, N., Suzuki, N., Yamagishi, M., Mizuno, J., Shoji, S., & Ohara, O. (2011). Single cell analysis of the proinflammatory responses of mast cell by a real time secretion assay. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 843-845). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).